Novosti
Khirurgii
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Year 2012 Vol. 20 No 6

EXPERIMENTAL SURGERY

I.V. MAIBORODIN, V.V. MOROZOV, JA.V. NOVIKOVA, V.A. MATVEEVA, L.V. ARTEMYEVA, A.L. MATVEEV, S.V. HOMENIUK, S.V. MARCHUKOV

ACCELERATION OF DEVELOPMENT OF POSTOPERATIVE GRANULATIONS AFTER ADMINISTRATION OF MESENCHYMAL STEM CELLS NEAR THE THROMBOSED VEIN IN EXPERIMENT

Center of Modern Medical Technology of the Institute of Chemical Biology and Fundamental Medicine of Siberian branch of RAS , Novosibirsk, The Russian Federation

Objectives. To estimate the possibility of paravasal application of the autologous multipotent mesenchymal stem cells of a bone marrow origin (AMMSCBMO) for the blood flow restoration in a thrombosed vein in experiment.
Methods. The morphological results of the AMMSCBMO introduction with GFP transfected gene and DAPI cellular nuclei additionally marked with a dye near the thrombosed vein were studied by means of the fluorescence microscopy.
Results. Many vessels consisting of the specifically fluorescent cells were found out in the place of a surgical intervention performed at thrombosis modeling. The restoration of the blood flow in the thrombosed main vein is always the result of thrombolysis. The signs of AMMSCBMO embedding into the wall of thrombosed vessels havent been found. The recanalization of the blood clot and formation of collaterals havent been detected as well. At thrombosis modeling in experiment? thrombosis of the small branches occurs by introduction of thrombin and ligation of the main vein. In them restoration of blood flow occurs by involving introduced AMMSCBMO or through a blood clot recanalization or through the obliteration of thrombosed vessels and formation of new ones. Thus the restoration of the blood flow in the tissue region of the affected vein is quicker probability of development of tissue hypoxemic necroses and other complications decreases. Gradually introduced AMMSCBMO and the structures generated with their participation, are extruded by own cells an organism-recipient.
Conclusions. At introduction of AMMSCBMO in paravasal cellulose of the main thrombosed vein, they take part in restoration of the tissue blood flow in the region of the thrombosed vein, and also in development of granulations in the place of the surgical intervention performed at thrombosis modeling.

Keywords: multipotent mesenchymal stem cells, venous thrombosis, recanalization of blood clot, angiogenesis, restoration of blood flow
p. 12 -19 of the original issue
References
  1. Shevchenko IuL, Stoiko IuM, Lytkina MI. Osnovy klinicheskoi flebologii [Fundamentals of Clinical Phlebology]. Moscow, RF: Meditsina, 2005. 312 p.
  2. Gu Y, Qi L, Zhang J, Guo L, Li J, Zhang S, Yu H, Li X, Cui S, Chen B, Wu Y, Tong Z, Wang Z. Middle-term outcome of autologous bone marrow mononuclear cells transplantation for treatment of lower limb ischemia. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Mar;23(3):34144. [Article in Chinese]
  3. Meng QY, Li XQ, Yu XB, Lei FR, Jiang K, Li CY. Transplantation of VEGF165-gene-transfected endothelial progenitor cells in the treatment of chronic venous thrombosis in rats. Chin Med J (Engl). 2010 Feb 20;123(4):47177.
  4. Tong Z, Gu Y, Zhang J, Li J, Zhang S, Wang Z. Changes of endothelial progenitor cells in rats after bone-marrow stimulation. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008 Oct;22(10):121821. [Article in Chinese]
  5. Chen YK, Jiang XM, Gong JP. Recombinant human granulocyte colony-stimulating factor enhanced the resolution of venous thrombi. J Vasc Surg. 2008 May;47(5):1058-65.
  6. Santo SD, Tepper OM, von Ballmoos MW, Diehm N, Volzmann J, Baumgartner I, Kalka C. Cell-based therapy facilitates venous thrombus resolution. Thromb Haemost. 2009 Mar;101(3):46064.
  7. Wessler S, Reimer SM, Sheps MC. Biological assay of a thrombosis inducing activity in human serum. J Appl Physiol. 1959 Nov;14:94346.
  8. Carmeliet P, Luttun A. The emerging role of the bone marrow-derived stem cells in (therapeutic) angiogenesis. Thromb Haemost. 2001 Jul;86(1):28997.
  9. Shi Q, Wu MH, Hayashida N, Wechezak AR, Clowes AW, Sauvage LR.
  10. Proof of fallout endothelialization of impervious Dacron grafts in the aorta and inferior vena cava of the dog. J Vasc Surg. 1994 Oct;20(4):54656.
  11. Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, Ishida A, Fujita Y, Kothari S, Mohle R, Sauvage LR, Moore MA, Storb RF, Hammond WP. Evidence for circulating bone marrow-derived endothelial cells. Blood. 1998 Jul 15;92(2):36267.
  12. Maiborodin IV, Iakimova NV, Matveeva VA, Pekarev OG, Maiborodina EI, Pekareva EO, Tkachuk OK. Morfologicheskii analiz rezul'tatov vvedeniia autologichnykh stvolovykh stromal'nykh kletok kostnomozgovogo proiskhozhdeniia v rubets matki krys [Morphological analysis of implantation results of autologous bone marrow stromal stem cells in rat uterine scar]. Morfologiia. 2010;138(6):4755
  13. Maiborodin IV, Iakimova NV, Matveeva VA, Pekarev OG, Maiborodina EI, Pekareva EO. Angiogenez v rubtse matki krys posle vvedeniia autologichnykh mezenkhimal'nykh stvolovykh kletok kostnomozgovogo proiskhozhdeniia [Angiogenesis in rat uterine scar after implantation of autologous bone marrow mesenchymal stem cells]. Biul Eksperim Biologii i Meditsiny. 2010;150(12):70511.
  14. Maiborodin I, Yakimova N, Matveeva V, Shevela A, Maiborodina E, Pekareva, Tkachuk O. Angiogenesis in rat uterine scar after introduction of autological mesenchymal stem cells of bone marrow origin. J Biomedical Science and Engineering. 2011;4(3):16472.
  15. Maiborodin IV, Kolesnikov IS, Sheplev BV, Ragimova TM, Shevela AI, Kovyntsev AN, Kolmakova IA, Pritchina IA, Kozlova EV, Voitovich AB, Kovyntsev DN. Granulematoznoe vospalenie posle primeneniia preparatov fibrina [Granulomatous inflammation after treatment of fibrin]. Morfol Vedomosti. 2007;1(3-4):11618.
  16. 15. Maiborodin IV, Kolesnikov IS, Sheplev BV, Ragimova TM, Kovyntsev AN, Kovyntsev DN, Shevela AI. Morfologiia podlezhashchikh tkanei desny posle dental'noi implantatsii s primeneniem preparatov fibrina [The morphology of the underlying gingival tissues after dental implantation by fibrin preparations]. Stomatologiia. 2009;88(1):913.
Address for correspondence:
630090, Rossiyskaya Federatsiya, g. Novosibirsk, pr. akad. Lavrenteva, d 8, Institut himicheskoy biologii i fundamentalnoy meditsinyi SO RAN, Tsentr novyih meditsinskih tehnologiy, laboratoriya stvolovoy kletki,
e-mail: imai@mail.ru.
Maiborodin Igor Valentinovich
Information about the authors:
Maiborodin I.V. Doctor of medical sciences, professor, leading researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Morozov V.V. Doctor of medical sciences, professor, leading researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Novikova JA.V. Candidate of medical sciences, researcher of the invasive medical technologies laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Matveeva V.A. Candidate of biological sciences, senior researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Artemyeva L.V. Leading engineer of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Matveev A.L. Post-graduate student of the molecular microbiology chair of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Homeniuk S.V. Researcher of the invasive medical technologies laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Marchukov S.V. Post-graduate student of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Contacts | ©Vitebsk State Medical University, 2007