Novosti
Khirurgii

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Year 2013 Vol. 21 No 4

EXPERIMENTAL SURGERY

V.D. LUTSENKO, A.A. MIGUNOV, T.N. TATYANENKO, E.B. SUCHALKIN, S.N. GONTAREV

APPLICATION OF BIOPOLYMERIC MATERIALS AT HOLLOW ORGAN PERFORATION IN EXPERIMENT

National Research University “Belgorod State Research University”

Objective. To study the effectiveness of biopolymer materials application in hollow organs perforation in the experiment.
Materials. Experiment is performed on 63 white mature rats. Simulation of gastric ulcers was carried out by a modified method of Okabe, to simulate the perforation a perforating puncture of gastric wall (D 0.1 cm) was made. Animals were divided into 3 groups (23, 25 and 15 animals). In the 1st group of animals the plasty of perforation is carried out by a membrane “Kollost”, in the 2nd group of animals a mesh endoprosthesis “Prolene” with coverage of omentum has been used. Animals of the 3d group are operated with application of traditional method – sutured by double-row stitches. Animals are disaffiliated with experiment on 7th, 14th, 21st, 30th, 60th, 90th and 120th days.
Results. On the 7th day it has been revealed that a connective tissue capsule of collagen fibers begins to form around the mesh. Epithelialization of ulcers is completed by the 30th day, a mess is fully encapsulated to the 60th day. Thus, in the area of perforation a positive reaction of connective tissue elements to the foreign synthetic material and minimization of adhesive process at cover of omentum have been revealed. On the 30th day the membrane “Kollost” in closing of ulcer defects promoted to the restoration of the organ wall and stimulated the scarring processes at the expense of specific protein-collagen. The density of protein structure allowed to delimit the lumen of the hollow organ from the abdominal cavity tightly. On the 60th day in suturing by double-row stitches the changes analogous to the healing of ulcers with biopolymer materials application have been revealed. In the long-term period the marked deformity and stricture formation are noted in the area of operations.
Conclusion. Application of biopolymeric materials accelerates the regenerative process of tissues in the operation zone, the tissues deformation and luminal constriction of organ are not occurred.

Keywords: perforation of a hollow organ, operative treatment, biopolymeric materials, morphological changes
p. 10 – 15 of the original issue
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Address for correspondence:
308036, Rossiiskaia Federatsiia, g. Belgorod, ul. Gubkina, 46, FGAOU VPO « Belgorodskii issledovatel'skii universitet», kafedra obshchei khirurgii meditsinskogo fakul'teta,
e-mail: Lutsenko@gb2bel.ru,
Lutsenko Vladimir Dmitrievich
Information about the authors:
Lutsenko V.D. MD, professor, a head of the general surgery chair with the course of topographic anatomy and operative surgery of the medical faculty of FSAEE HPE "Belgorod State National Research University".
Migunov A.A. PhD, senior lecturer of the general surgery chair with the course of topographic anatomy and operative surgery of the medical faculty of FSAEE HPE "Belgorod State National Research University ".
Tatyanenko T.N.PhD, associate professor of the general surgery chair with the course of topographic anatomy and operative surgery of the medical faculty of FSAEE HPE "Belgorod State National Research University ".
Suchalkin E.B. assistant of the general surgery chair with the course of topographic anatomy and operative surgery of the medical faculty of FSAEE HPE "Belgorod State National Research University ".
Gontarev S.N. MD, professor of the general surgery chair with the course of topographic anatomy and operative surgery of the medical faculty of FSAEE HPE "Belgorod State National Research University ".  
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