Novosti
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Year 2015 Vol. 23 No 3
SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY
K.S. KOMISSAROV 1, V.S. PILOTOVICH 1, M.Y. YURKEVICH 1, M.V. DMITRIEVA 2, M.M. ZAFRANSKAYA 1
TECHNICAL FEATURES OF EXPERIMENTAL MODEL OF ACUTE RENAL ISCHEMIA-REPERFUSION INJURY
SEE "Belarusian Medical Academy of Post-
Graduate Education" 1,
ME "Minsk City Pathology Bureau"2
Minsk
The Republic of Belarus
Objectives. To evaluate the reproducibility of renal ischemia-reperfusion injury model in rats using transabdominal access with bilateral renal pedicles clamping within 50 minutes.
Methods. The experiment was conducted in sexually mature, albino, purebred male rats (n=8, 270-310 g). The referent group (n=4) consisting of intact animals comparable in sex and weight was formed. The laboratory animals were anesthetized with transperitoneal sodium thiopental injection. Bilateral renal clamping through median laparotomy was used to reproduce ischemia-reperfusion (I/R) model with 50 min ischemia, the reperfusion period – 24 and 48 hours. The control (Sham) group (n=2) was allocated, the rats underwent to all stages of the surgery except renal clamping. Efficiency of ischemia development was evaluated by measuring serum creatinine and urea determined by the standard methodology and histological changes of renal tissue.
Results. None of the animals died during the post-operative period. Increasing of the serum creatinine level by 1,5 folds and more in comparison with referent data was fixed in 5 out of 6 laboratory animals whereas serum creatinine was not different from normal level in Sham group. Histological evaluation of the renal tissue revealed the different changes indicating the development of proximal tubular injury in 100% of rats.
Conclusion. The obtained results testify to the development of acute renal failure in 83,3% rats after 50 min. ischemia and independently on 24 or 48 hours reperfusion that allows using transabdominal access with bilateral renal clamping for the reproduction of the ischemia-reperfusion model of acute kidney injury.
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Address for correspondence:
220013, Respublika Belarus,
g. Minsk, ul. P.Brovki, d. 3,
GUO "Belorusskaya meditsinskaya akademiya
poslediplomnogo obrazovaniya",
kafedra urologii i nefrologii,
tel. office: 375 172 92 35 34,
e-mail: kirill_ka@tut.by
Komissarov Kirill Sergeevich
Komissarov K.S. PhD, an associate professor of the urology and nephrology chair of SBE "Belarusian Medical Academy of Post-graduate Education".
Pilotovich V.S. MD, professor of the urology and nephrology chair of SBE "Belarusian Medical Academy of Post-graduate Education".
Yurkevich M.Y. A researcher of the immunological group of SBE "Belarusian Medical Academy of Post-graduate Education".
Dmitrieva M.V. A physician-pathologist of ME "Minsk City Pathology Bureau".
Zafranskaya M.M. PhD, associate professor of the immunological group of SBE "Belarusian Medical Academy of Post-graduate Education".
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