Novosti
Khirurgii

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Year 2021 Vol. 29 No 2

GENERAL & SPECIAL SURGERY

I.V. SHIPITSYNA, E.V. OSIPOVA

SPECIES COMPOSITION OF ASSOCIATIONS AND RELATIONSHIPS BETWEEN MICROORGANISMS ISOLATED FROM OSTEOMYELITIS FOCUS

National Ilizarov Scientific Center for Traumatology and Orthopedics, Kurgan,
The Russian Federation

Objective. To determine the species composition of the associations isolated from osteomyelitis foci and to study the character of associate relationships based on the biofilm-forming ability data.
Methods. The microbiological study included clinical isolates (n=184) obtained from associations (n=88) during primary inoculations from wounds and fistulas of patients (n= 88) with chronic osteomyelitis of long tubular bones. In order to obtain an associative biofilm in vitro, the cultures of competing bacterial strains were daily mixed in 1:1 ratio. The biofilms were grown on the surface of polystyrene plates with subsequent determination of the level of biofilm formation in 24 and 48 hours. The coefficient of relationship (CR) was calculated to evaluate the synergistic, neutral and antagonistic relationships between bacteria in the biofilms.
Results. The associations of staphylococcus with gram-negative bacteria were most frequently recovered from osteomyelitis foci. On the 1st day of the experiment, 38,6 % of associations had a moderate biofilm-forming ability, and besides, associations of gram-positive + gram-negative bacteria were observed in 36,4%; 42,1% of associations had a low biofilm-forming ability; 19,3% – had a high biofilm-forming ability. After 48 hours the percentage of mild adhesive strains remained at the same level – 38,6%, as for the low adhesive ones it decreased to 36,4%, high adhesive – increased up to 25%. Most bacterial associations manifested antagonistic relationships. Synergism in biofilm-formingby the association of S. aureus + P. aeruginosa was observed in 2 cases,while the level of film-forming was high as on the first and the second day of the study. In several associations it transformed from antagonistic to synergistic or neutral relationships.
Conclusion. It has ben established that among the identified associations, the largest specific weight falls on the associations of gram-positive + gram-negative bacteria , while S. aureus is one of the most common components. These associations were noted to have high and mild activity of biofilm -forming on the surface of polystyrene plates. Relationships between the microorganisms isolated from osteomyelitis foci in associations, as a rule, are antagonistic.

Keywords: osteomyelitis, associations of bacteria, biofilms, antagonism, synergism, coefficient of relationship
p. 183-190 of the original issue
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Address for correspondence:
640014, Russian Federation,
Kurgan, M.Ulyanovoy Str., 6,
National Ilizarov Scientific Center for Traumatology and Orthopedics, Ministry of Health of Russia, Research Clinical Laboratory
of Microbiology and Immunology
tel.mob. +7 909 179-26-01,
å-mail vschimik@mail.ru
Shipitsyna Irina V.
Information about the authors:
Shipitsyna Irina V., PhD, Researcher of the Research Clinical Laboratory of Microbiology and Immunology, National Ilizarov Scientific Center for Traumatology and Orthopedics of the Ministry of Health of the Russian Federation, Kurgan,Russian Federation.
http://orcid.org/0000-0003-2012-3115
Osipova Elena V., PhD, Senior Researcher of the Research Clinical Laboratory of Microbiology and Immunology, National Ilizarov Scientific Center for Traumatology and Orthopedics of the Ministry of Health of the Russian Federation, Kurgan,Russian Federation.
http://orcid.org/0000-0003-2408-4352
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