Novosti
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This journal is indexed in Scopus |
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Year 2021 Vol. 29 No 3
TRAUMATOLOGY AND ORTHOPEDICS
S.V. ZYBLEVA, S.L. ZYBLEV
IMMUNOLOGICAL PREDICTORS OF RENAL GRAFT REJECTION IN THE EARLY POSTOPERATIVE PERIOD
Republican Research Center for Radiation Medicine and Human Ecology, Gomel,
The Republic of Belarus
Objective. To determine the immunological predictors of renal graft rejection in the early postoperative period.
Methods. Three groups were formed out of the 197 renal graft recipients. The group PGF (n=101) was made up of patients with satisfactory primary graft function. The group PGD (n = 82) included patients with primary graft dysfunction without episodes of rejection. The group RGR (n=14) consisted of patients with primary dysfunction and renal graft rejection. On the 7th day after transplantation the early kidney graft function was assessed on the basis of serum creatinine levels. When the serum creatinine value was lower than 300 μmol/L the function was considered to be primary, at a creatinine concentration was equal to or higher than 300 μmol/L, as well as in the case of need for maintenance dialysis on the first week after transplantation, the state was classified as the renal graft dysfunction. In the early postoperative period, the number of LIN-HLA-DR+ dendritic cells with the LIN- HLA-DR+CD11c+CD123- and LIN-HLA-DR+CD11c-CD123+ phenotypes in the fluid from the drainage installed to the kidney graft during surgery was determined. Predictive characteristics of the mDC and pDC levels in the drainage fluid were determined to predict renal graft rejection, and diagnostic capability of this indicator were identified.
Results. It has been revealed that renal graft rejection is characterized by a significant growth of the total number of dendritic cells in the drainage fluid, mainly due to myeloid ones. Predictive characteristics were determined by the level of myeloid and plasmacytoid dendritic cells in the drainage fluid. The cut-off point of the level of myeloid dendritic cells was determined at the level of 60.32%, and for plasmacytoid dendritic cells it corresponded to 39.68%.
Conclusion. With the level of myeloid dendritic cells in the drainage fluid greater or equal 60.32%, and plasmacytoid cells lower or equal 39.68%, renal graft rejection is predicted with a sensitivity of 99% and 93%, respectively, and a specificity of 89% and 91%, respectively.
- Zhuang Q, Lakkis FG. Dendritic cells and innate immunity in kidney transplantation. Kidney Int. 2015 Apr;87(4):712-18. doi: 10.1038/ki.2014.430
- Morelli AE. Dendritic cells of myeloid lineage: the masterminds behind acute allograft rejection. Curr Opin Organ Transplant. 2014 Feb;19(1):20-27. doi: 10.1097/MOT.0000000000000039
- Ezzelarab M, Thomson AW. Tolerogenic dendritic cells and their role in transplantation. Semin Immunol. 2011 Aug;23(4):252-63. doi: 10.1016/j.smim.2011.06.007
- Belz GT, Nutt SL. Transcriptional programming of the dendritic cell network. Nat Rev Immunol. 2012 Jan 25;12(2):101-13. doi: 10.1038/nri3149
- Morelli AE, Thomson AW. Tolerogenic dendritic cells and the quest for transplant tolerance. Nat Rev Immunol. 2007 Aug;7(8):610-21. doi: 10.1038/nri2132
- Solari MG, Thomson AW. Human dendritic cells and transplant outcome. Transplantation. 2008 Jun 15;85(11):1513-22. doi: 10.1097/TP.0b013e318173a768
- Matta BM, Castellaneta A, Thomson AW. Tolerogenic plasmacytoid DC. Eur J Immunol. 2010 Oct;40(10):2667-76. doi: 10.1002/eji.201040839
- Maldonado RA, von Andrian UH. How tolerogenic dendritic cells induce regulatory T cells. Adv Immunol. 2010;108:111-65. doi: 10.1016/B978-0-12-380995-7.00004-5
- Pulendran B, Tang H, Manicassamy S. Programming dendritic cells to induce T(H)2 and tolerogenic responses. Nat Immunol. 2010 Aug;11(8):647-55. doi: 10.1038/ni.1894
- Cantaluppi V, Dellepiane S, Tamagnone M, Medica D, Figliolini F, Messina M, Manzione AM, Gai M, Tognarelli G, Ranghino A, Dolla C, Ferrario S, Tetta C, Segoloni GP, Camussi G, Biancone L. Neutrophil gelatinase associated lipocalin is an early and accurate biomarker of graft function and tissue regeneration in kidney transplantation from extended criteria donors. PLoS One. 2015 Jun 30;10(6):e0129279. doi: 10.1371/journal.pone.0129279. eCollection 2015.
- Zhou H, Wu L. The development and function of dendritic cell populations and their regulation by miRNAs. Protein Cell. 2017 Jul;8(7):501-513. doi: 10.1007/s13238-017-039
- Nosik AV, Korotkov SV, Smolnikova VV, Hrynevich VYU, Dmitrieva MV, Dolgolikova AA, Pikirenia II, Krivenko SI, Kalachik OV, Shcherba AE, Rummo OO. The value of assessment of lymphocytes subpopulations numbers in peripheral blood for diagnosis of cellular rejection after kidney transplantion. Novosti Khirurgii. 2019;27(4):409-20. doi: 10.18484/2305-0047.2019.4.409 (In Russ.)
- Colonna M, Trinchieri G, Liu YJ. Plasmacytoid dendritic cells in immunity. Nat Immunol. 2004 Dec;5(12):1219-26. doi: 10.1038/ni1141
- Zuidwijk K, de Fijter JW, Mallat MJ, Eikmans M, van Groningen MC, Goemaere NN, Bajema IM, van Kooten C. Increased influx of myeloid dendritic cells during acute rejection is associated with interstitial fibrosis and tubular atrophy and predicts poor outcome. Kidney Int. 2012 Jan;81(1):64-75. doi: 10.1038/ki.2011.289
246000, Republic of Belarus,
Gomel, Ilyich Str., 290,
Republican Research Center
for Radiation Medicine and Human Ecology
tel. mobile: +375 44 547-69-85,
e-mail: zyb-svetlana@yandex.by,
Zybleva Svetlana V.
Zybleva Svetlana V., PhD, Immunologist, Scientific Secretary, Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus.
https://orcid.org/0000-0003-3061-5324
Zyblev Sergey L., PhD, Associate Professor, Surgeon of the Surgery Unit (Transplantation, Reconstructive and Endocrine Surgery), Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus.
https://orcid.org/0000-0002-0968-6630
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