Novosti
Khirurgii

 This journal is
indexed in Scopus
journal cover
android icon Android Applicaion

Year 2021 Vol. 29 No 3

ONCOLOGY

S.V. VOLKOV, S.L. LOBANOV, B.S. ERDINEYEVA, A.A. DUTOVA

THE ROLE OF THE INTESTINAL MICROBIOTA IN THE COLON CANCER DIAGNOSTICS

Chita State Medical Academy, Chita,
The Russian Federation

Objective. To determine the composition of the the parietal microflora of the colon under the condition of a tumor process and to assess the possibility of using these data from this study in the diagnosis of the colon cancer.
Methods. The main group included patients (n=75) operated on in the Trans-Baikal Regional Oncology Center for the colon cancer. Among them are 38 men and 37 women aged 20 to 75 years. Control group consisted of 25 patients.
The material of the main group, biopsies of tumor tissue and visually unchanged colon mucosa was taken intraoperatively during tumor removal. In patients of the control group, the material was taken during the colonoscopy. process. The detection of Helicobacter pylori in the biopsy of the mucous membrane was carried out by determining the urease activity, as well as by the method of polymerase chain reaction. The composition of the intestinal microbiota was determined by the bacteriological method.
Results. As a result of comparison of the colon microbiota of the cancer patients and the control group, statistically significant differences in the quantitative composition of Lactobacillus spp., Bifidobacterium spp., Bacteroides spp., Clostridium spp., Enterococcus spp., E. coli (typical), E. coli (lactose-negative), Enterobacteriaceae, Staphylococcus spp. (CNS), Candida spp. were revealed. Moreover, a clear tendency of changes in the level of certain microorganisms (Bifidobacterium spp., E. coli (typical), Clostridium sp.) was detected when comparing the mucous membrane of a healthy person, unchanged mucous membrane of a patient with colorectal cancer, tumor tissue. A statistically significant dependence of the level of microorganisms on the sex of a patient, as well as the form of tumor growth, was revealed.
Conclusion. Thus, a reliable correlation between the clinical and morphological characterization of the colon malignant tumor and the composition of the intestinal microbiota has been revealed. The microbiological features established within in the course of the study, which arise precisely under the conditions of colon cancer, can be used as an additional diagnostic marker in the diagnosis of a malignant process.

Keywords: colon cancer, Helicobacter pylori, intestinal microbiota, colon biopsy, tumor tissue
p. 326-333 of the original issue
References
  1. Dai Z, Zhang J, Wu Q, Chen J, Liu J, Wang L, Chen C, Xu J, Zhang H, Shi C, Li Z, Fang H, Lin C, Tang D, Wang D. The role of microbiota in the development of colorectal cancer. Int J Cancer. 2019 Oct 15;145(8):2032-41. doi: 10.1002/ijc.32017
  2. Hernández-Luna MA, López-Briones S, Luria-Pérez R. The four horsemen in colon cancer. J Oncol. 2019 Sep 29;2019:5636272. doi: 10.1155/2019/5636272
  3. Bahmani S, Azarpira N, Moazamian E. Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742. Turk J Gastroenterol. 2019 Sep;30(9):835-42. doi: 10.5152/tjg.2019.18451
  4. Hooi JKY, Lai WY, Ng WK, Suen MMY, Underwood FE, Tanyingoh D, Malfertheiner P, Graham DY, Wong VWS, Wu JCY, Chan FKL, Sung JJY, Kaplan GG, Ng SC. Global prevalence of helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology. 2017 Aug;153(2):420-29. doi: 10.1053/j.gastro.2017.04.022
  5. Butt J, Epplein M. Helicobacter pylori and colorectal cancer-A bacterium going abroad? PLoS Pathog. 2019 Aug 8;15(8):e1007861. doi: 10.1371/journal.ppat. 1007861
  6. Volkov SV, Lobanov SL. Criteria of clinical aggressiveness of colorectal cancer. Zabajkal Med Vestn. 2018;(2):69-76. http://zabmedvestnik.ru/arhiv-nomerov/nomer-2-za-2018-god/kriterii-klinicheskoj-agressivnosti-kolorektalnogo-raka (In Russ.)
  7. Kountouras J, Kapetanakis N, Polyzos SA, Katsinelos P, Gavalas E, Tzivras D, Zeglinas C, Kountouras C, Vardaka E, Stefanidis E, Kazakos E. Active Helicobacter pylori infection is a risk factor for colorectal mucosa: early and advanced colonic neoplasm sequence. Gut Liver. 2017 Sep 15;11(5):733-34. doi: 10.5009/gnl16389
  8. Sivan A, Corrales L, Hubert N, Williams JB, Aquino-Michaels K, Earley ZM, Benyamin FW, Lei YM, Jabri B, Alegre ML, Chang EB, Gajewski TF. Commensal Bifidobacterium promotes antitumor immunity and facilitates anti-PD-L1 efficacy. Science. 2015 Nov 27;350(6264):1084-89. doi: 10.1126/science.aac4255
  9. Wei Y, Li F, Li L, Huang L, Li Q. Genetic and biochemical characterization of an exopolysaccharide with in vitro antitumoral activity produced by Lactobacillus fermentum YL-11. Front Microbiol. 2019 Dec 17;10:2898. doi: 10.3389/fmicb. 2019.02898
  10. Dai Z, Zhang J, Wu Q, Chen J, Liu J, Wang L, Chen C, Xu J, Zhang H, Shi C, Li Z, Fang H, Lin C, Tang D, Wang D. The role of microbiota in the development of colorectal cancer. Int J Cancer. 2019 Oct 15;145(8):2032-41. doi: 10.1002/ijc. 32017
  11. Zamani S, Taslimi R, Sarabi A, Jasemi S, Sechi LA, Feizabadi MM. Enterotoxigenic Bacteroides fragilis: a possible etiological candidate for bacterially-induced colorectal precancerous and cancerous lesions. Front Cell Infect Microbiol. 2020 Jan 17;9:449. doi: 10.3389/fcimb.2019.00449
  12. Sheng QS, He KX, Li JJ, Zhong ZF, Wang FX, Pan LL, Lin JJ. Comparison of gut microbiome in human colorectal cancer in paired tumor and adjacent normal tissues. Onco Targets Ther. 2020 Jan 21;13:635-46. doi: 10.2147/OTT.S218004
  13. Zorron Cheng Tao Pu L, Yamamoto K, Honda T, Nakamura M, Yamamura T, Hattori S, Burt AD, Singh R, Hirooka Y, Fujishiro M. Microbiota profile is different for early and invasive colorectal cancer and is consistent throughout the colon. J Gastroenterol Hepatol. 2019 Oct 23;35(3):433-37. doi: 10.1111/jgh.14868
  14. Butt J, Varga MG, Blot WJ, Teras L, Visvanathan K, Le Marchand L, Haiman C, Chen Y, Bao Y, Sesso HD, Wassertheil-Smoller S, Ho GYF, Tinker LE, Peek RM, Potter JD, Cover TL, Hendrix LH, Huang LC, Hyslop T, Um C, Grodstein F, Song M, Zeleniuch-Jacquotte A, Berndt S, Hildesheim A, Waterboer T, Pawlita M, Epplein M. Serologic response to helicobacter pylori proteins associated with risk of colorectal cancer among diverse populations in the United States. Gastroenterology. 2019 Jan;156(1):175-86.e2. doi: 10.1053/j.gastro.2018.09.054
  15. Changxi C, Mao Y, Du J, Xu Y, Zhu Z, Cao H. Helicobacter pylori infection associated with an increased risk of colorectal adenomatous polyps in the Chinese population. BMC Gastroenterol. 2019 Jan 21;19(1):14. doi: 10.1186/s12876-018-0918-4
Address for correspondence:
672090, Russian Federation,
Chita, Gor’kii Str., 39a,
Chita State Medical Academy
of the Ministry of Health of Russia,
Oncology Department
tel.: +7 924 370-99-65,
e-mail: vsv_19@mail.ru,
Volkov Stepan V.
Information about the authors:
Volkov Stepan V., Assistant, the Oncology Department, Chita State Medical Academy of the Ministry of Health of Russia, Chita, Russian Federation.
https://orcid.org/0000-0002-8696-9562
Lobanov Sergey L., MD, Professor, Head of the Department of Faculty Surgery with the Course of Urology, Chita State Medical Academy of the Ministry of Health of Russia, Chita, Russian Federation.
http://orcid.org/0000-0003-1665-3754
Dutova Anastasiya A., PhD, Senior Researcher, the Laboratory of Molecular Genetics, RI of the Molecular Medicine, Chita State Medical Academy of the Ministry of Health of Russia, Chita, Russian Federation.
http://orcid.org/0000-0001-8285-6061
Erdineyeva Beligma S., PhD, Assistant, the Department of Microbiology, Virology, Immunology, Chita State Medical Academy of the Ministry of Health of Russia, Chita, Russian Federation.
http://orcid.org/0000-0003-3035-7900
Contacts | ©Vitebsk State Medical University, 2007-2023