Novosti
Khirurgii

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Year 2021 Vol. 29 No 4

GENERAL & SPECIAL SURGERY

R.E. KALININ, I.A. SUCHKOV, A.V. SCHULKIN, E.A. KLIMENTOVA, A.A. EGOROV

INFLUENCE OF VARIOUS SURGICAL INTERVENTIONS ON VASCULAR WALL APOPTOSIS INDICES IN PATIENTS WITH ATHEROSCLEROSIS OF PERIPHERAL ARTERIES

I.P. Pavlov Ryazan State Medical University, Ryazan,
Russian Federation

Objective. Assessment of apoptosis proteins Bcl-2 and Bax indices before and after the open vascular reconstructions and X-ray endovascular interventions in patients with the peripheral atherosclerosis of the arteries of the lower extremities.
Methods. The study included patients (n=40) with the peripheral atherosclerosis of the lower extremities arteries (stage III-IV) disease who underwent open surgery – group A, and patients (n=40) who underwent endovascular interventions – group B. Two apoptosis proteins were analyzed in blood serum: Bax and Bcl-2 initially before surgery, on the 1st day and after 1 month. The reference values of the studied parameters were determined in 40 healthy volunteers.
Results. Initial Bax protein index – 27.1 ng / ml (ð<0,001) were elevated in patients of group A and reduced Bax protein – 4.4 ng / ml (p=0.00008) in patients of group B compared with indices of healthy volunteers (16.5 ng / ml, 5.3 ng / ml, respectively). On the 1st day after the interventions in the patients of the operative groups there was an increasing tendency for Bax index to 35.6 ng / ml (p<0.001) – group A, to 25.6 ng/ml (p<0.001) – group B. By the end of 1st month, Bax was increased to 28 ng/ml (p<0.001) in patients of group A and Bcl-2 was reduced to 3.0 ng / ml (p=0.039) compared to the initial indices; in patients of group B, only protein Bax – 23.9 ng / ml (p<0.001) was increased.
Conclusion. Open and endovascular intervention on the arteries of the lower extremities leads to the activation of the pro-apoptotic protein Bax on the 1st day after surgery. In the postoperative period the open reconstruction leads to an evaluation of the pro-apoptotic potential in comparison with endovascular intervention in the form of increasing Bax protein and reducing Bcl-2 by the end of the first month.

Keywords: apoptosis, peripheral arterial atherosclerosis, apoptosis proteins, Bc-l2, Bax
p. 420-425 of the original issue
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Address for correspondence:
390026, Russian Federation,
Ryazan, Vysokovoltnaya Str., 9,
Ryazan State Medical University,
the Department of Cardiovascular,
X-ray Endovascular,
Operative Surgery and Topographic Anatomy,
tel.: +7 903ì836-24-17,
e-mail: Suchkov_med@mail.ru,
Suchkov Igor A.
Information about the authors:
Kalinin Roman E., MD, Professor, Rector, Head of the Department of Cardiovascular, X-ray Endovascular, Operative Surgery and Topographic Anatomy, I.P. Pavlov Ryazan State Medical University, Ryazan, Russian Federation.
https://orcid.org/0000-0002-0817-9573
Suchkov Igor A., MD, Professor, Vice-Rector for Research and Innovative Development, Professor of the Department of Cardiovascular, X-ray Endovascular, Operative Surgery and Topographic Anatomy, I.P. Pavlov Ryazan State Medical University, Ryazan, Russian Federation.
https://orcid.org/0000-0002-1292-5452.
Shchul’kin Aleksey V., MD, Associate Professor of the Department of Pharmacology with the Course of Pharmacy of Continuing Professional Education Faculty, I.P. Pavlov Ryazan State Medical University, Ryazan, Russian Federation.
https://orcid.org/0000-0003-1688-0017
Klimentova Åmma A., PhD, Post-Graduate Student of the Department of Cardiovascular, X-ray Endovascular, Operative Surgery and Topographic Anatomy, I.P. Pavlov Ryazan State Medical University, Ryazan, Russian Federation.
https://orcid.org/0000-0003-4855-9068
Egorov Andrey A., MD, Associate Professor of the Department of Cardiovascular, X-ray Endovascular, Operative Surgery and Topographic Anatomy, I.P. Pavlov Ryazan State Medical University, Ryazan, Russian Federation.
https://orcid.org/0000-0003-0768-7602
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