This journal is
indexed in Scopus
Year 2011 Vol. 19 No 3
LYZIKOV A.A., VOROPAEV E.V., OSIPOV V.A., PECHENKIN A.A.
MODELING HIGH RISK CONDITIONS OF INFECTIOUS COMPLICATIONS IN EXPERIMENT
Objectives. To create the experimental model of the subcultural bacteremia to study biological reactions of the vascular conduits on the presence of pathogenic microorganisms in the dose insufficient for sepsis development.
Methods. Experimental investigations were carried out on 30 out bred dogs with an average weight 15,3±3,7 kg. Aorto-femoral bypass with a combined conduit was performed in all of them. Modeling of high-risk conditions of postoperative septic complications by a single intravenous injection of S.aureus was performed in 20 dogs before the operation. 10 other animals were used for control and were not infected.
Results. The leucocytes level and clinical parameters in the experimental group didnt reliably differ from ones in the control group. For 3–5 days 14 out of 20 animals of the experimental group died because of the massive hemorrhage from the anastomoses. PCR analysis was carried out: in some animals together with the control strain of Staphylococcus a normal micro flora was identified as well as other types of Staphylococcus.
Conclusions. The presence of subcultural bacteriemia doesn t cause clinical and laboratory manifestations of sepsis but result in complications of conduit survival similar to the septic ones. Development of infectious complications doesnt depend on the dosage of pathogenic agent.
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