Novosti
Khirurgii
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Year 2012 Vol. 20 No 1

EXPERIMENTAL SURGERY

I.O. POKHODENKO-CHUDAKOVA 1, E.V. MAKSIMICH 1, S.F. KURALENYA 2

COMPARATIVE EVALUATION OF MORPHOLOGICAL CHANGES AT DIFFERENT VARIANTS OF INJECTION OF 2% SOLUTION OF LYDOKAIN HYDROCHLORIDE IN EXPERIMENT

EE Belarusian State Medical University 1,
ME Minsk consultational -diagnostics center 2,
the Republic of Belarus

Objectives. To study the morphological changes of the parenchymatous organs of the laboratory animals at various variants of the local anesthetic 2% lidocaine hydrochloride solution in the experiment at its frequent repeated injections.
Methods. The research was carried out on 27 white mouse males. All animals were divided into two series (16 and 11 animals) in which the anesthetic was injected 5 times a day in every 3-4 days (in the dose 50 mg/kg of the animal weight). Anesthetic injection was made in the maxillofacial area in the animals of the first series (the variant of the conducting mandibular anesthesia outside the mouth); intraperitoneal introduction of the drugs was made in the second series animals.
Results. Lethality after 2% lidocaine hydrochloride solution injection made up 37,5%. There were no lethal outcomes in the second series. Significant incidence of toxic manifestation from the central nervous system was established in the experimental animals of the first series; it can be explained by peculiarity of the head and neck vascularization and innervation. The most evident morphological changes of the parenchymatous organs were noted in the animals of the first series.
Conclusions. Morphological changes of the parenchymatous organs of the laboratory animals depend on the region of 2% lidocaine hydrochloride solution injection; it should be taken into consideration when conducting preclinical studies of drugs, used in the dental practice.

Keywords: morphological changes, toxicity, lidocaine
p. 16 19 of the original issue
References

1.Krzhechkovskaia VV, Vakhtangishvili RSh. Lekarstvennye sredstva v anesteziologii. Mestnye anestetiki. [Drugs in anesthesiology. Local anesthetics]. Rostov-na-Donu, RF: Feniks; 2006. 192 p.
2.Poniakina ID. Vyiavlenie povyshennoi chuvstvitel'nosti organizma k stomatologicheskim preparatam in vitro [Detection of hypersensitivity to dental preparations in vitro]. Stomatologiia dlia vsekh. 2004;(3):4450.
3.Violin BV, Ignatova ID, Ignatova AD. Izuchenie toksichnosti lekarstvennoi formy florfenikola dlia peroral'nogo primeneniia v opytakh na laboratornykh zhivotnykh [The study of the florfenikol toxic dosage for oral administration in experiments on laboratory animals] . Sb nauch tr VGNKI. 2006;67. p. 17581.
4.Bircher AJ, Messmer SL, Surber C, Rufli T. Delayed-type hypersensitivity to subcutaneous lidocaine with tolerance to articaine: confirmation by in vivo and in vitro tests. Contact Dermatitis. 1996;34(6):38789.
5.Astakshin EA. Rukovodstvo po laboratornym zhivotnym i al'ternativnym modeliam v biomeditsinskikh tekhnologiiakh [Guidance on laboratory animals and alternative models in biomedical technology]. Karkishchenko NN, Gracheva SV, editors. Moscow, : Standartinform; 2010. 344 p.
6.Kutsenko SA. Osnovy toksikologii: uchebnoe posobie [Foundation of toxicology: a tutorial]. Saint-Petersburg, RF: Voenno-meditsinskaia akademiia im SM Kirova; 2002. 395 p.
7.Khibriev RU. Rukovodstvo po eksperimental'nomu (doklinicheskomu) izucheniiu novykh farmakologicheskikh veshchestv [Guidelines for experimental (preclinical) studies of new pharmacological agents]. Moscow, RF: Meditsina; 2005. 832 p.
8.Vorob'ev AL. Klinicheskaia anatomiia i operativnaia khirurgiia golovy i shei [Clinical anatomy and operative surgery of the head and neck]. Saint-Petersburg, : Elbi-SPb; 2008. 256 p.
9.Stoliarenko PIu. Istoriia sozdaniia lidokaina [History of the lidocaine]. Samara, RF: SamGMU; 2001. 36 p.
10.Denisov SD, Morozkina TS. Trebovanija k nauchnomu jeksperimentu s ispol'zovaniem zhivotnyh [Requirements for scientific experiment with animals]. Zdravoohranenie. 2001;(4):4042.
11.Belousov JuB, Gurevich KG. Klinicheskaja farmakokinetika. Praktika dozirovanija lekarstv: spec. vypusk serii Racional'na farmakoterapija [Clinical pharmacokinetics. The practice of dispensing drugs: spec. produce a series of Rational pharmacotherapy]. Moscow, RF: Litterra; 2005. 288 p.
12.Malamed SF. Allergicheskie i toksicheskie reakcii na mestnye anestetiki [Allergic and toxic reactions to local anesthetics]. Klin Stomatologija. 2004;(4):2630.
13.Gazhva SI, Pichugin VV, Sokolov VV. Metody obezbolivanija v stomatologii pri serdechno-sosudistyh zabolevanijah [Methods of anesthesia in dentistry in patients with cardiovascular diseases]. Nizhny Novgorod, RF: NGMA; 2008. 124 p.

Address for correspondence:
220116, Respublika Belarus', g. Minsk, pr-t. Dzerzhinskogo, d. 83, UO Belorusskii gosudarstvennyi meditsinskii universitet, kafedra khirurgicheskoi stomatologii,
e-mail: ip-c@yandex.ru,
Pokhodenko-Chudakova Irina Olegovna
Information about the authors:
Pokhodenko-Chudakova I.O. Doctor of medical sciences, professor, head of the surgical dentistry chair of EE Belarusian State Medical University.
Maksimovich E.V. Assistant of the surgical dentistry chair of EE Belarusian State Medical University.
Kuralenya S.F. head of the laboratory of clinical pathologic histology of ME Minsk Consultational -Diagnostics Center.
Contacts | ©Vitebsk State Medical University, 2007