Novosti
Khirurgii

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Year 2013 Vol. 21 No 2

EXPERIMENTAL SURGERY

I.V. MAIBORODIN, A.I. SHEVELA, V.A. MATVEEVA, M.N. DROVOSEKOV, M.I. BARANNICK, I.V. KUZNETSOVA

ANGIOGENESIS IN GRANULATION TISSUE AFTER IMPLANTATION OF POLYHYDROXYALKANOATE WITH MESENCHYMAL STEM CELLS

Center of Modern Medical Technology of the Institute of Chemical Biology and Fundamental Medicine of Siberian branch of RAS, Novosibirsk,
The Russian Federation

Objectives. To estimate the processes occurring in various tissues after implantation of polymeric films from polyhydroxyalkanoate (PHA) with the adsorbed autologous multipotent mesenchymal stem cells of the bone marrow origin (AMMSCBM) in experiment.
Methods. The processes occurring in the rat abdominal cavity, muscular and subcutaneous adipose tissues after implantation of PHA with the adsorbed AMMSCBM and transfected DNA of fluorescent protein GFP were studied by the methods of light microscopy.
Results. It was revealed that after implantation of PHA with AMMSCBM the increase of blood vessels number in the surrounding tissues was occurred as a result of neoangiogenesis processes. In this case AMMSCBM don’t migrate and don’t collapse in the site of administration, but form the blood vessels on account of differentiation of their structures in cells. Processes of angiogenesis in tissues around PHA in turn lead to formation of large number of blood vessels in the granulations surrounded the implanted foreign body, larger volume of granulations and thicker capsule, delimiting polymer in the future. However it is possible that formation of a thick capsule with fibrous signs after implantation of PHA with AMMSCBM is considered as the unfavorable prognostic signs indicating to high probability of various complications development afterwards. Administrated AMMSCBM are replaced eventually by their own recipient cells.
Conclusions. The implantation of PHA with AMMSCBM leads to the formation of large number of blood vessels in the granulations forming round the implanted foreign body, larger volume of granulations and thicker capsule delimiting polymer in the future.

Keywords: multipotent mesenchymal stromal cells, polyhydroxyalkanoate, implantation, angiogenesis, granulomatous inflammations
p. 29 – 36 of the original issue
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Address for correspondence:
630090, Rossiiskaia Federatsiia, g. Novosibirsk, pr. akad. Lavrent'eva, d. 8, Institut khimicheskoi biologii i fundamental'noi meditsiny SO RAN, Tsentr novykh meditsinskikh tekhnologii, laboratoriia stvolovoi kletki,
e-mail: imai@mail.ru,
Maiborodin Igor' Valentinovich
Information about the authors:
Maiborodin I.V. MD, professor, leading researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
Shevela A.I. MD, professor, Honored Doctor of RF, deputy director of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
Matveeva V.A. PhD, a senior researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
Drovosekov M.N. PhD, an applicant for Doctor’s degree of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
Barannik M.I. PhD, an applicant for Doctor’s degree of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
Kuznetsova I.V. PhD, a researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk.
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