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Year 2015 Vol. 23 No 4


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SBEE HPE "Krasnoyarsk State Medical University named after professor V.F. Voino-Yasenetsky" 1,
DSBME "Krasnoyarsk Interregional Clinical Hospital 7" 2
The Russian Federation

Objectives. To evaluate the application effectiveness of directed flow of the ozone-oxygen gaseous mixture to a sanitation of pyogenic wounds in experiment.
Methods. An experimental study was conducted in 30 male rabbits of Soviet Chinchilla breed. Each individual was simulated a pyogenic wound via daily broth culture of methicillin resistant strain of Staphylococcus aureus. In the main group (n=10) within the whole diastasis preservation period the local treatment of edgar wouhds with ozone-oxygen gaseous mixture the sanitation by the original device was performed. In the comparison group (n=10) of the wounds was carried out in sealed plastic contour chambers filled with ozone-oxygen gaseous mixture. In the control group (n=10) a consistent antiseptic treatment of pyogenic wounds with 3% hydrogen peroxide solution and 0,01% solution miramistin has been cleansed.
Evaluation of the treatment effectiveness was performed clinically and histologically on the 1st, 7th, 30th days of the experiment.
Results. In the clinical evaluation of the local status prior to treatment, the wounds consisted of soft tissue defects with specific signs of inflammation and purulent discharge. The relevant intergroup differences of biopsy results of purulent wounds histologically after the simulation also havent been revealed. On the 7th day in the control group preparations pronounced signs of inflammation preserved, while in the main group preparations the transition to the proliferative phase was registered. On the 30th day the shaped scar was determined clinically at the site of the wound defect in animals of all groups. Histological assessment of biopsies of scarring in the wound treatment groups with the ozone-oxygen gaseous mixture the dense connective tissue without any signs of the inflammatory response has been revealed, while in the control one the loose connective tissue with inflammatory infiltration was visualized.
Conclusion. In using the directed flow of the ozone-oxygen gaseous mixture to sanitation of pyogenic wounds in experiment, the earlier cleansing and healing with the formation of connective tissue scar without any signs of inflammatory response have been registered.

Keywords: skin and soft tissue infections, pyogenic wounds, the ozone-oxygen gaseous mixture, ozone, cleansing, sanitation, healing
p. 372-378 of the original issue
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Address for correspondence:
660022, Russian Federation, g. Krasnoyarsk,
ul. Partizana Zheleznyaka, d. 1,
GBOU VPO "Krasnoyarskiy gosudarstvennyiy
meditsinskiy universitet imeni professora
V.F. Voyno-Yasenetskogo", kafedra
obschey khirurgii im. prof. M.I. Gulmana,
Vinnik Yuriy Semenovich
Information about the authors:
Vinnik Y.S. MD, an Honored Worker of Science of RF, an Honored Physician of Russia, a Head of the general surgery chair named after M.I.Gulman of SBEE HPE "Krasnoyarsk State University named after professor V.F. Voino-Yasenetsky".
Plakhotnikova A.M. A competitor of the general surgery chair named after M.I.Gulman of SBEE HPE "Krasnoyarsk State University named after professor V.F. Voino-Yasenetsky".
Kirichenko A.K. MD, a head of the pathologic anatomy chair named after P.G. Podzolkov of SBEE HPE "Krasnoyarsk State University named after professor V.F. Voino-Yasenetsky".
Kukonkov V.A. PhD, a head of the surgical department 2 of the district state budgetary medical establishment "Krasnoyarsk Interregional Clinical Hospital 7".
Teplyakova O.V. PhD, an associate professor of the general surgery chair named after M.I.Gulman of SBEE HPE "Krasnoyarsk State University named after professor V.F. Voino-Yasenetsky".
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