Novosti
Khirurgii
This journal is
indexed in Scopus



Year 2016 Vol. 24 No 6

GENERAL & SPECIAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2016.6.568   |  

V.K. OKULICH

ABZYME ACTIVITY OF IMMUNOGLOBULINS IN SURGICAL INFECTION

EE "Vitebsk State Medical University",
Vitebsk,
The Republic of Belarus

Objectives. To determine the role of proteolytic, DNAse, esculinase and urease activity of abzymes in clinical and laboratory picture and to assess the capacity to destroy exopolymeric matrix of biofilms in patients with surgical infection.
Methods. All the patients tested for abzyme activity were divided into the following groups: a group of persons with various chronic inflammatory processes; with spreading inflammatory processes; persons with local acute inflammatory processes, pyoinflammatory disease of the maxillofacial area, chronic osteomyelitis, cholecystitis, adenomatous goiter, gastroduodenitis and severe form of pneumonia. Control group consisted of donors of blood transfusion station and patients without pyoinflammatory processes. Proteolytic activity of immunoglobulin G in reactions with chromogenic substrates: benzoyl-arginine-p-nitroanilide (BAPNA), lysine-p-nitroanilide (LPA), arginine-proline-p-nitroanilide (APNA) and acetyl-aspartate-p-nitroanilide (AANA) was determined in patients with surgical infection and healthy donors.
Results. It was found out that the rate of BAPNA-amidase abzyme activity in patients with surgical infection was reliably higher, LPA- and APNA-amidase rate didnt differ and AANA-amidase rate was significantly lower than in donors. It was revealed that immunoglobulins derived from patients with surgical infection have the ability to destroy the exopolymeric matrix of the biofilm. Presence of reliably elevated levels of DNAse and esculinase activity of IgG in surgical patients with pyo-inflammatory processes in comparison with patients without suppurative complications was determined. Positive reliable correlation between type of causative agent and DNAse activity of IgG was revealed. The elevated levels of urease activity of IgG, derived from patients with gastroduodenitis, associated with H. pylori in comparison with patients without H. pylori infection was shown.
Conclusion. Obtained data concerning catalytic activity of IgG in patients with infectious process may be used for development of new diagnostic criteria and methods for prognosis of pyoinflammatory diseases.

Keywords: surgical infection, catalytic antibodies, abzymes, biofilm, proteolytic activity, diagnostic criteria, pyoinflammatory diseases
p. 568-578 of the original issue
References
  1. Kosinets AN, Kosinets VA, Struchkov IuV. Infektsiia v khirurgii [Infection in Surgery]: uchebnik. Minsk, RB: Bel Entsykl im P Bro?ki; 2012. 496 s.
  2. Generalov II. Abzimnaia aktivnost' immunoglobulinov [Abzyme activity immunoglobulin]. Vitebsk, RB: VGMU; 2000. 152 p.
  3. Leatnerbarrow RJ. Designer catalytic antibodies. Nature. 1989 Mar 16;338:206-207. doi: 10.1038/338206a0.
  4. Keinan E. Catalytic Antibodies. Germany: VCH-Wiley press; 2005. 586 .
  5. Odintsova ES, Parkhomenko TA, Kunder EV, Okulich VK, Zhil'tsov IV, Sen'kovich SA, i dr. DNK-gidrolizuiushchie IgG antitela iz krovi patsientov bakterial'nymi infektsionnymi zabolevaniiami [DNA-hydrolyzing antibody IgG from the blood of patients with bacterial infectious diseases]. Immunopatologiia Allergologiia Infektologiia. 2006;(2):23-31.
  6. Brown EL, Nishiyama Y, Dunkle JW, Aggarwal S, Planque S, Watanabe K, et al. Constitutive production of catalytic antibodies to a Staphylococcus aureus virulence factor and effect of infection. J Biol Chem. 2012 Mar 23;287(13):9940-51. doi: 10.1074/jbc.M111.330043.
  7. Paul S, Planque SA, Nishiyama Y, Hanson CV, Massey RJ. Nature and nurture of catalytic antibodies. Adv Exp Med Biol. 2012;750:56-75. doi: 10.1007/978-1-4614-3461-0_5.
  8. Jerne NK. Towards a network theory of the immune system. Ann Immunol (Paris). 1974 Jan;125C(1-2):373-89.
  9. Davey ME, O'toole GA. Microbial biofilms: from ecology to molecular genetics. Microbiol Mol Biol Rev. 2000 Dec;64(4):847-67.
  10. Dongari-Bagtzoglou A. Pathogenesis of mucosal biofilm infections: challenges and progress. Expert Rev Anti Infect Ther. 2008 Apr;6(2):201-8. doi: 10.1586/14787210.6.2.201.
  11. Chebotar' IV, Maianskii AN, Konchakova ED, Lazareva AV, Chistiakova VP. Antibiotikorezistentnost' bioplenochnykh bakterii [Antibiotic resistance of biofilm bacteria]. Klin Mikrobiologiia i Antimikrob Khimioterapiia. 2012;14(1):51-58.
  12. Mal'tsev SV, Mansurova GSh. Chto takoe bioplenka [What is biofilm]. Prirodnaia Meditsina: Klinicheskie Issledovaniia. 2013;1:86-89.
  13. Frimel G, red. Immunologicheskie metody [Immunological methods]: monografiia. Tarasova AP, per. s nem. Moscow, SSSR: Meditsina; 1987. 472 p.
  14. Okulich VK, Sen'kovich SA, Plotnikov FV, Kabanova AA, Matskevich EL. Rol' abzimnoi aktivnosti immunoglobulinov klassa G u patsientov s khirurgicheskoi infektsiei [Role of abzyme activity of immunoglobulin G in patients with surgical infection. Khirurgiia Vostochnaia Evropa. 2013;(2):6-14.
  15. Pollack SJ, Jacobs JW, Schultz PG. Selective chemical catalysis by an antibody. Science. 1986 Dec 19;234(4783):1570-73.
Address for correspondence:
210023, Republic of Belarus,
Vitebsk, pr. Frunze, 27, EE "Vitebsk
State Medical University",
department of clinical microbiology,
Tel.: +375 212 06.12.37
E-mail: vokul@mail.ru
Okulich Vitaly Konstantinovich
Information about the authors:
Okulich V.K. PhD, Ass. Professor of department of clinical microbiology, EE "Vitebsk State Medical University".
Contacts | ©Vitebsk State Medical University, 2007