Novosti
Khirurgii
This journal is
indexed in Scopus



Year 2017 Vol. 25 No 3

SCIENTIFIC PUBLICATIONS
EXPERIMAENTAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2017.3.233   |  

I.V. MAIBORODIN 1, V.V. MOROZOV 1, A.A. ANIKEEV 1, N.F. FIGURENKO 1, R.V. MASLOV 1, G.A.CHASTIKIN 1, V.A. MATVEEVA 1, V.I.MAIBORODINA 2

MACROPHAGE REACTION TO MULTIPOTENT MESENCHYMAL STROMAL CELLS INTRODUCTION INTO SURGICAL TRAUMA SITE IN RATS

FSBES "Institute of Chemical Biology and Fundamental Medicine",
of Russian Academy of Sciences, Siberian Branch
FSBES "Institute of Molecular Pathology and Pathomorphology",
Novosibirsk
The Russian Federation

Objectives. To prove the possibility of phagocytosis by macrophage of autologic multipotent mesenchymal stromal cells of the bone marrow origin (AMMSCBMO) injected into the surgical site.
Methods. In 12 rats the macrophage reaction 2 weeks after the injection of AMMSCBMO with a transfected GFP-gene and additionally stained by Vybrant-CM-Dil cellular membranes into the site of a surgical trauma were investigated by light fluorescent microscopy with the application of monoclonal antibodies to CD68-antigen.
Results. AMMSCBMO injected into the site of a surgical intervention are partially phagocytized by macrophages from the tissues in the site of injection. Phagocytosis of AMMSCBMO is accompanied by rapid degradation of GFP-protein whereas Vybrant-CM-Dil either isnt destroyed by enzymes of lisosomes or degrades very slowly. The accumulation of dye in macrophages has occured and, as a result, the phagocytes receive the ability to an intensive fluorescence in irradiation them by ultraviolet light with filter for rhodamine. AMMSCBMO and their fragments can get into the blood and lymphatic vessels and appear, at least, in the regional inguinal lymph nodes (ILN); it is possible to capture AMMSCBMO and their colored detritus from the lumen of the vessels by perivascular macrophages located in distant tissues.
In the cortex of ILN after AMMSCBMO injection in the site of lymph collection, in the sites corresponding to the location of lymphoid nodules, both the number and size of collections of large luminous macrophages, and their number in such collections have been increased. Some phagocytes from lymphoid nodules of ILN contain specifically stained AMMSCBMO fragments.
Conclusion. The macrophage phagocytosis of AMMSCBMO from the tissues in site of injection and vascular bed has been proved. Presence of macrophages with AMMSCBMO detritus in lymphoid nodules of ILN can serve the indirect confirmation of high probability of initiation of the humoral immune defense against the antigens of injected AMMSCBMO.

Keywords: multipotent mesenchymal stromal cells, lymph nodes, macrophages, macrophage fluorescence, humoral immune, lymphoid nodules, phagocytes
p. 233-241 of the original issue
References
  1. Varol C, Mildner A, Jung S. Macrophages: development and tissue specialization. Annu Rev Immunol. 2015;33:643-75. doi: 10.1146/annurev-immunol-032414-112220.
  2. Okabe Y, Medzhitov R. Tissue biology perspective on macrophages. Nat Immunol. 2016;17(1):9-17. doi: 10.1038/ni.3320.
  3. Maiborodin IV, Matveeva VA, Maslov RV, Onoprienko NV, Kuznetsova IV, Chastikin GA. Fluorestsiruiushchie makrofagi v limfaticheskikh uzlakh posle primeneniia mul'tipotentnykh mezenkhimal'nykh stromal'nykh kletok s transfitsirovannym genom GFP [Fluorescent macrophages in the lymph nodes after the use of multipotent mesenchymal stromal cells with a transfected GFP gene]. Novosti Khirurgii. 2014;22(5):526-32. doi: 10.18484/2305-0047.2014.5.526.
  4. Möllerherm H, Köckritz-Blickwede von M, Branitzki-Heinemann K. Antimicrobial activity of mast cells: role and relevance of extracellular DNA traps. Front Immunol. 2016;7:265. doi: 10.3389/fimmu.2016.00265.
  5. Pinke KH, Lima HG, Cunha FQ, Lara VS. Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1. Immunobiology. 2016 Feb;221(2):220-27. doi: 10.1016/j.imbio.2015.09.004.
  6. Levin R, Grinstein S, Canton J. The life cycle of phagosomes: formation, maturation, and resolution. Immunol Rev. 2016 Sep;273(1):156-79. doi: 10.1111/imr.12439.
  7. Naqvi AR, Fordham JB, Nares S. MicroRNA target Fc receptors to regulate Ab-dependent Ag uptake in primary macrophages and dendritic cells. Innate Immun. 2016 Oct;22(7):510-21. doi: 10.1177/1753425916661042.
  8. Mendes-Jorge L, Ramos D, Luppo M, Llombart C, Alexandre-Pires G, Nacher V, et al. Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5997-6005. doi: 10.1167/iovs.09-3515.
  9. Mitchell AJ, Pradel LC, Chasson L, Van Rooijen N, Grau GE, Hunt NH, et al. Technical advance: autofluorescence as a tool for myeloid cell analysis. J Leukoc Biol. 2010 Sep;88(3):597-603. doi: 10.1189/jlb.0310184.
  10. Li F, Yang M, Wang L, Williamson I, Tian F, Qin M, et al. Autofluorescence contributes to false-positive intracellular Foxp3 staining in macrophages: a lesson learned from flow cytometry. J Immunol Methods. 2012 Dec 14;386(1-2):101-7. doi: 10.1016/j.jim.2012.08.014.
  11. Potter KA, Simon JS, Velagapudi B, Capadona JR. Reduction of autofluorescence at the microelectrode-cortical tissue interface improves antibody detection. J Neurosci Methods. 2012 Jan 15;203(1):96-105. doi: 10.1016/j.jneumeth.2011.09.024.
  12. Gavrilin VN, Shkurupii VA. Vliianie nakopleniia polivinilpirrolidona v sinusoidal'nykh kletkakh pecheni na kharakter toksicheskogo povrezhdeniia organa [The effect of the accumulation of polyvinyl pyrrolidone in sinusoidal liver cells on the nature of toxic organ damage]. Biul SO RAMN. 1995;(2):24-28.
Address for correspondence:
630090, Russian Federation, Novosibirsk,
Acad. Lavrentiev Ave 8,
FSBES "Institute of Chemical Biology
and Fundamental Medicine of
the SB RAS, stem cell laboratory
Tel.: 8-913-753-07-67,
E-mail: imai@mail.ru,
Igor V. Maiborodin
Information about the authors:
Maiborodin I.V. MD, Professor, Chief researcher of the stem cell laboratory of FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Morozov V.V. MD, Professor, Head of laboratory of invasive medical technologies, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Anikeev A.A. PhD, applicant for Doctors degree of FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Figurenko N.F. PhD, Applicant for Doctors degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Maslov R.V. PhD, Applicant for Doctors degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
ChastikinG.A. PhD, Applicant for Doctors degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Matveeva V.A. PhD (Biology), Senior Researcher of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Maiborodina V.I. MD, Leading Researcher of the laboratory of pathology ultrastructural foundations, FSBES "Institute of Molecular Pathology and Pathomorphology".
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