This journal is
indexed in Scopus
Year 2017 Vol. 25 No 3
I.V. MAIBORODIN 1, V.V. MOROZOV 1, A.A. ANIKEEV 1, N.F. FIGURENKO 1, R.V. MASLOV 1, G.A.CHASTIKIN 1, V.A. MATVEEVA 1, V.I.MAIBORODINA 2
MACROPHAGE REACTION TO MULTIPOTENT MESENCHYMAL STROMAL CELLS INTRODUCTION INTO SURGICAL TRAUMA SITE IN RATS
FSBES "Institute of Chemical Biology and Fundamental Medicine",
of Russian Academy of Sciences, Siberian Branch
FSBES "Institute of Molecular Pathology and Pathomorphology",
The Russian Federation
Objectives. To prove the possibility of phagocytosis by macrophage of autologic multipotent mesenchymal stromal cells of the bone marrow origin (AMMSCBMO) injected into the surgical site.
Methods. In 12 rats the macrophage reaction 2 weeks after the injection of AMMSCBMO with a transfected GFP-gene and additionally stained by Vybrant-CM-Dil cellular membranes into the site of a surgical trauma were investigated by light fluorescent microscopy with the application of monoclonal antibodies to CD68-antigen.
Results. AMMSCBMO injected into the site of a surgical intervention are partially phagocytized by macrophages from the tissues in the site of injection. Phagocytosis of AMMSCBMO is accompanied by rapid degradation of GFP-protein whereas Vybrant-CM-Dil either isn’t destroyed by enzymes of lisosomes or degrades very slowly. The accumulation of dye in macrophages has occured and, as a result, the phagocytes receive the ability to an intensive fluorescence in irradiation them by ultraviolet light with filter for rhodamine. AMMSCBMO and their fragments can get into the blood and lymphatic vessels and appear, at least, in the regional inguinal lymph nodes (ILN); it is possible to capture AMMSCBMO and their colored detritus from the lumen of the vessels by perivascular macrophages located in distant tissues.
In the cortex of ILN after AMMSCBMO injection in the site of lymph collection, in the sites corresponding to the location of lymphoid nodules, both the number and size of collections of large luminous macrophages, and their number in such collections have been increased. Some phagocytes from lymphoid nodules of ILN contain specifically stained AMMSCBMO fragments.
Conclusion. The macrophage phagocytosis of AMMSCBMO from the tissues in site of injection and vascular bed has been proved. Presence of macrophages with AMMSCBMO detritus in lymphoid nodules of ILN can serve the indirect confirmation of high probability of initiation of the humoral immune defense against the antigens of injected AMMSCBMO.
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630090, Russian Federation, Novosibirsk,
Acad. Lavrentiev Ave 8,
FSBES "Institute of Chemical Biology
and Fundamental Medicine of
the SB RAS, stem cell laboratory
Igor V. Maiborodin
Maiborodin I.V. MD, Professor, Chief researcher of the stem cell laboratory of FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Morozov V.V. MD, Professor, Head of laboratory of invasive medical technologies, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Anikeev A.A. PhD, applicant for Doctor’s degree of FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Figurenko N.F. PhD, Applicant for Doctor’s degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Maslov R.V. PhD, Applicant for Doctor’s degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
ChastikinG.A. PhD, Applicant for Doctor’s degree of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Matveeva V.A. PhD (Biology), Senior Researcher of the stem cell laboratory, FSBES "Institute of Chemical Biology and Fundamental Medicine", the Russian Academy of Sciences, Siberian Branch.
Maiborodina V.I. MD, Leading Researcher of the laboratory of pathology ultrastructural foundations, FSBES "Institute of Molecular Pathology and Pathomorphology".