Novosti
Khirurgii
This journal is
indexed in Scopus



Year 2017 Vol. 25 No 3

GENERAL & SPECIAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2017.3.273   |  

I.V. SHIPITSYNA, E.V. OSIPOVA, L.V. ROZOVA

ADHESIVE POTENTIAL OF CLINICAL STRAINS OF ENTEROBACTER CLOACAE ISOLATED FROM THE WOUNDS OF PATIENTS WITH CHRONIC OSTEOMYELITIS AND THEIR SENSITIVITY TO ANTIMICROBIAL PREPARATIONS

FSB "Russian State Scientific Centre "Restorative
traumatology and orthopaedics" named after the academician G.A.Ilizarov of the Ministry of Health of the Russian Federation",
Kurgan
The Russian Federation

Objectives. To study adhesive properties of Enterobacter cloacae strains isolated from the wounds of patients with chronic osteomyelitis and to evaluate the sensitivity to the antibacterial preparations used in clinical practice.
Methods. The clinical strains of Enterobacter cloacae (n=18) isolated preoperatively from the fistulas and from the inflammatary focus within the surgery in patients (n=18) with chronic osteomyelitis of long tubular bones for the period of 2015-2016 were studied. The biofilm-forming potential of monocultures of strain E. cloacae and their associations obtained in vitro were investigated: E. cloacae+ S. aureus (n=6), E. cloacae+ S. epidermidis (n=5), E. cloacae+P. aeruginosa (n=3). The sensitivity of isolated E. cloacae strains to the antibacterial preparations used in clinical practice was determined.
Results. Strains of Enterobacter cloacae were characterized by average biofilm-forming potential, and on the 2nd day of the experiment the level of biofilm formation was reliably higher than daily values. In co-cultivation of E. cloacae and S. aureus bacteria had led to the reduction of biofilm formation. In co-cultivation of E. cloacae, P. aeruginosa, E. cloacae and S. epidermidis bacteria the potential of biofilm formation was higher comparing with pure cultures of the studied strains.
The performed study demonstrated 100% resistance of E. cloacae bacteria to ampicillin, ceftriaxone and amoksiklav. Enterobacter bacteria can produce the enzymes of ß-lactamase hydrolyzed ß-lactam antibiotics, and therefore there is a high percentage of resistant strains. Imipenem and meropenem were the most effective to E. cloacae strains among antibacterial preparations.
Conclusion. The ability of clinical strains of E. cloacae to adhesion by forming a biofilm, as well as the high resistance to antimicrobial drugs testify to a significant pathogenic potential that should be taken into account in diagnosing and treating chronic osteomyelitis.

Keywords: Enterobacter cloacae, chronic osteomyelitis, biofilm, adhesion, antibiotic resistant, wounds, high resistance
p. 273-278 of the original issue
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Address for correspondence:
640014, Russia, Kurgan, M. Ulyanova str.,
6, FSB "Russian State Scientific Centre
"Restorative traumatology and orthopaedics" named after the academician G.A.Ilizarov of the Ministry of Health of the Russian Federation,
laboratory of microbiology and immunology, Tel.: 8-909-179-26-01,
E-mail: IVSchimik@mail.ru.
Irina V. Shipitsyna
Information about the authors:
Shipitsyna I.V. PhD (Biology), Researcher of laboratory of microbiology and immunology, FSB "Russian State Scientific Centre "Restorative traumatology and orthopaedics" named after the academician G.A.Ilizarov of the Ministry of Health of the Russian Federation.
Osipova E.V. PhD (Biology), Leading Researcher of laboratory of microbiology and immunology of FSB "Russian State Scientific Centre
"Restorative traumatology and orthopaedics" named after the academician G.A.Ilizarov of the Ministry of Health of the Russian Federation.
Rozova L.V. Junior researcher of laboratory of microbiology and immunology, of FSB "Russian State Scientific Centre "Restorative traumatology and orthopaedics" named after the academician G.A.Ilizarov of the Ministry of Health of the Russian Federation.
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