Novosti
Khirurgii
This journal is
indexed in Scopus



Year 2018 Vol. 26 No 2

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2018.2.135   |  

M.G. RYABKOV 2, E.B. KISELEVA 1, N.D. GLADKOVA 1, M.S. BALEEV 2, E.L. BEDERINA 2, E.E. LUKOYANYCHEV 2, A.A. MIRONOV 1, I.L. DEZORTSEV 2, V.V. BESCHASTNOV 2

THE ROLE OF INTRAMURAL BLOODSTREAM DYSFUNCTION IN THE DEVELOPMENT OF SMALL INTESTINE ISCHEMIC NECROSIS

Nizhny Novgorod State Medical Academy 1,
City Clinical Hospital 30, Nizhny Novgorod 2,
The Russian Federation

Objective. To study experimentally the mechanisms of intramural bloodstream dysfunction and small bowel necrosis development in case of the acute mesenteric ischemia.
Methods. Acute complete mesenteric ischemia was modelled in laboratory animals by ligating a. mesenterica cranialis. The OCT-based microangiography technique was used to monitor bowel microcirculation till the visual signs of its non-viability appeared. Then the bowel was resected, the degree of ischemic lesion was histologically evaluated.
Results. In occlusion of mesenteric arteries by the moment when macroscopic nonviability signs appear, the ischemic damage without necrosis spreads out to 22.2% of the bowel length and mucous tunic necrosis and transmural necrosis spread out to 38.1% and 39.7% of the bowel length correspondingly. While comparing the histological preparations and OCT images, the features of OCT-microangiograms were described in different degrees of ischemic lesion and bowel necrosis. According to OCT-microangiography, in 60.3% of the wall of the ischemic intestine the number of functioning vessels remained normal. The decrease in the length of functioning vessels (by 5.6%, p=0.029), the total area of the vascular bed (by 4.5%, p=0.032) and the average vascular density (by 5.1%, p=0.001) occurred only in the intestinal wall with transmural necrosis. The mechanism of the superficial bowel necrosis development was a decrease in the proportion of small diameter vessels (p=0.029) against the background of the preserved microcirculation.
Conclusions. The attempt to recommence hemocirculation in the bowel arteries which are traditionally considered empty is the basis of modern surgical treatment of the mesenteric ischemia. However, the authors came to the conclusion that for 38% of ischemic bowel the blood supply remains at a normal level till necrosis development. This fact seems important for improving results of surgical treatment of acute mesenteric ischemia because it gives the opportunity to save a part of the bowel without thrombectomy. In these cases the OCT-based microangiography method can be an effective noninvasive tool for functioning blood vessels visualization and control of treatment results.

Keywords: acute mesenteric ischemia, intestinal infarction, mesenteric arterial occlusion, microcirculation, optical coherence tomography, OCT-based microangiography, mesenteric revascularization
p. 135-145 of the original issue
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Address for correspondence:
605157, The Russian Federation,
Nizhny Novgorod, Berezovskaya Str., 85,
City Clinical Hospital 30
of Moscow District,
Surgical Unit 2,
Tel.: +7 905 012-21-50,
e-mail: maxim-ryabkov@yandex.ru,
Ryabkov Maxim G.
Information about the authors:
Ryabkov Maxim G., MD, Associate Professor, Consultant of the Surgical Unit 2, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0002-9555-190X
Kiseleva Elena B., PhD, Researcher of the Laboratory of Studying the Optical Properties of Tissues of SRI of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0003-4769-417X
Gladkova Natalia D., MD, Professor, Deputy Director of SRI of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Russian Federation.
http://orcid.org/0000-0002-8386-7157
Baleev Mixail S., Surgeon, the Surgical Unit 2, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0001-6943-9757
Bedrina Evgenia L., Pathologist, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, the Chief Out-Of-Staff Specialist (Pathologist) of the Department of Health, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0001-5368-8396
Lukoyanychev Egor E., PhD, Surgeon, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0001-6392-2692
Mironov Andrey A., PhD, Senior Researcher of the Central Research Laboratory, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0001-7387-2860
Dezortsev Ilya L., Surgeon, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0003-2715-8599
Beschastnov Vladimir V., MD, Associate Professor, Surgical Unit N2, Consultant of the Surgical Unit 2, City Clinical Hospital 30 of Moscow District, Nizhny Novgorod, Russian Federation.
http://orcid.org/0000-0002-9332-3858
Contacts | ©Vitebsk State Medical University, 2007