This journal is
indexed in Scopus
Year 2019 Vol. 27 No 2
V.N. NIKITIN 1, V.L. POLUEKTOV 1, T.P. KHRAMYKH 1, L.O. BARSKAYA 1, E.A. ZABLOTSKAIA 2, V.A. PUTINTSEV 1, V.P. EFANOV 1
FIRST RESULTS OF AN ORIGINAL METHOD APPLICATION OF EXPERIMENTAL STOMACH ULCER MODELING
Omsk State Medical University 1,
Omsk Regional Clinical Medical Sanitary Unit ¹ 9 2, Omsk,
The Russian Federation
Objective. To work out and to test experimentally the method for modeling gastric ulcer in small laboratory animals.
Methods. The experiment included 38 white mongrel rats. Mini-laparotomy was performed, stomach was taken out into the operating field. 70% solution of the acetic acid was introduced into the submucosal layer from the side of the stomach lumen using the device for intra-organ intraluminal injections. The animals were excluded from the experiment on the 3rd, 5th, 7th day after the operation, the area of ulcerative defect with microscopic assessment of tissue and cellular composition in the area of ulcerative defect was determined.
Results. In two animals, when injected into the submucosal layer of the stomach wall 0.06 ml of 70% acetic acid, there was a total necrosis of all layers with concurrent phlegmon of the stomach; they died on the 2nd day because of the generalized peritonitis on the background of perforation of the stomach necrotic wall. The median area of gastric ulcer was: with the introduction of 0.04 ml after 3 days – 1.88 cm2, after 7 days – 0.34 cm2; with the introduction of 0.03 ml – 0.94 cm2 and 0.19 cm2, respectively; with the introduction of 0.02 ml – 0.63 cm2 and 0.16 cm2, respectively; with the introduction of 0.01 ml – 0.31 cm2 and 0.02 cm2, respectively. It is morphologically confirmed that the bottom of the ulcer is the muscle layer of the stomach. Thus, the threshold dose of the injected 70% acid solution (0.06 ml) was revealed, the excess of which results in total necrosis of the stomach wall, as well as the prospects for a possible relationship between the volume of the damaging factor, the size of the emerging ulcer defect and the healing time of the latter.
Conclusions. The proposed method of modeling gastric ulcers is reproducible and can be recommended for use in experimental surgery.
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644043, The Russian Federation,
Omsk, Lenin Str., 12,
Omsk State Medical University,
Department of Faculty Surgery, Urology.
Tel.: +7 3812 35-91-30,
Vyacheslav N. Nikitin
Nikitin Vyacheslav N., PhD, Associate Professor of the Department of Faculty Surgery, Urology, Omsk State Medical University, Omsk, Russian Federation.
Poluektov Vladimir L., MD, Professor, Head of the Department of Faculty Surgery, Urology, Omsk State Medical University, Omsk, Russian Federation.
Khramykh Tatyana P., MD, Professor, Head of the Department of Topographic Anatomy and Operative Surgery, Omsk State Medical University, Omsk, Russian Federation.
Barskaya Lyubov O., Assistant of the Department of Topographic Anatomy and Operative Surgery, Omsk State Medical University, Omsk, Russian Federation.
Zablotskaia Elena A., Morbid Anatomist, Omsk Regional Clinical Medical Sanitary Unit ¹ 9, Omsk, Russian Federation.
Putintsev Vladislav A., 5-Year Medical Student, Omsk State Medical University, Omsk, Russian Federation.
Efanov Vladimir P., 5-Year Medical Student, Omsk State Medical University, Omsk, Russian Federation.