Novosti
Khirurgii
This journal is
indexed in Scopus



Year 2019 Vol. 27 No 2

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2019.2.131   |  

V.N. NIKITIN 1, V.L. POLUEKTOV 1, T.P. KHRAMYKH 1, L.O. BARSKAYA 1, E.A. ZABLOTSKAIA 2, V.A. PUTINTSEV 1, V.P. EFANOV 1

FIRST RESULTS OF AN ORIGINAL METHOD APPLICATION OF EXPERIMENTAL STOMACH ULCER MODELING

Omsk State Medical University 1,
Omsk Regional Clinical Medical Sanitary Unit 9 2, Omsk,
The Russian Federation

Objective. To work out and to test experimentally the method for modeling gastric ulcer in small laboratory animals.
Methods. The experiment included 38 white mongrel rats. Mini-laparotomy was performed, stomach was taken out into the operating field. 70% solution of the acetic acid was introduced into the submucosal layer from the side of the stomach lumen using the device for intra-organ intraluminal injections. The animals were excluded from the experiment on the 3rd, 5th, 7th day after the operation, the area of ulcerative defect with microscopic assessment of tissue and cellular composition in the area of ulcerative defect was determined.
Results. In two animals, when injected into the submucosal layer of the stomach wall 0.06 ml of 70% acetic acid, there was a total necrosis of all layers with concurrent phlegmon of the stomach; they died on the 2nd day because of the generalized peritonitis on the background of perforation of the stomach necrotic wall. The median area of gastric ulcer was: with the introduction of 0.04 ml after 3 days 1.88 cm2, after 7 days 0.34 cm2; with the introduction of 0.03 ml 0.94 cm2 and 0.19 cm2, respectively; with the introduction of 0.02 ml 0.63 cm2 and 0.16 cm2, respectively; with the introduction of 0.01 ml 0.31 cm2 and 0.02 cm2, respectively. It is morphologically confirmed that the bottom of the ulcer is the muscle layer of the stomach. Thus, the threshold dose of the injected 70% acid solution (0.06 ml) was revealed, the excess of which results in total necrosis of the stomach wall, as well as the prospects for a possible relationship between the volume of the damaging factor, the size of the emerging ulcer defect and the healing time of the latter.
Conclusions. The proposed method of modeling gastric ulcers is reproducible and can be recommended for use in experimental surgery.

Keywords: gastric ulcer, experimental modeling, acetic acid, rats, intraluminal injection, mini-laparotomy
p. 131-137 of the original issue
References
  1. Brzozowski T. Experimental production of peptic ulcer, gastric damage and cancer models and their use in pathophysiological studies and pharmacological treatment- Polish achievements. J Physiol Pharmacol. 2003 Dec;54(Suppl 3):99-126. http://www.jpp.krakow.pl/journal/archive/12_03_s3/articles/07_article.html
  2. Agbaje EO, Doe YP. Gastric and duodenal antiulcer effects of aqueous bark extract of Dialium guineense Wild. (Fabaceae) and the possible mechanisms in laboratory models. J Phytopharmacol. 2015;4(5):268-75. http://www.phytopharmajournal.com/Vol4_Issue5_06.pdf
  3. Bahadır FE, Özbeyli D, Mardinoğlu G, Ozbeyli D, Temiz F, M. Sağanak, H. Şahin, M. Yüksel, F.Ercan, B. Yeğen. Regular swimming before the induction of ulcer ameliorates oxidative gastric damage in rats: the anxiolytic effect of exercise. MMJ. 2016;29(3):135-44. doi: 10.5472/MMJoa.2903.01
  4. Manjegowda SB, Rajagopal HM, Dharmesh SM. Polysaccharide of Black cumin (Nigella sativa) modulates molecular signaling cascade of gastric ulcer pathogenesis. Int J Biol Macromol. 2017 Aug;101:823-36. doi: 10.1016/j.ijbiomac.2017.03.093
  5. de Oliveira Cabral C, Campos A, da Silva LM, Boeing T, de Andrade SF, Filho VC, Nesello LÂN. Gastroprotective potential of methanolic extract and dimethyl cardamonin from Campomanesia reitziana fruits in mice. Naunyn Schmiedebergs Arch Pharmacol. 2017 Jun;390(6):661-66. doi: 10.1007/s00210-017-1369-0
  6. Okabe S, Amagase K. An overview of acetic acid ulcer models-the history and state of the art of peptic ulcer research. Biol Pharm Bull. 2005 Aug;28(8):1321-41. doi: 10.1248/bpb.28.1321
  7. Lutsenko VD, Migunov AA, Tatyanenko TN, Suchalkin EB, Gontarev SN. Application of biopolymeric materials at hollow organ perforation in experiment. Novosti Khirurgii. 2013;21(4):10-15. doi: 10.18484/2305-0047.2013.4.10 (in Russ.)
  8. Berenguer B, Sánchez LM, Quílez A, López-Barreiro M, de Haro O, Gálvez J, Martín MJ. Protective and antioxidant effects of Rhizophora mangle L. against NSAID-induced gastric ulcers. J Ethnopharmacol. 2006 Jan 16;103(2):194-200. doi: 10.1016/j.jep.2005.08.029
  9. Oh TY, Ahn GJ, Choi SM, Ahn BO, Kim WB. Increased susceptibility of ethanol-treated gastric mucosa to naproxen and its inhibition by DA-9601, an Artemisia asiatica extract. World J Gastroenterol. 2005 Dec 21;11(47):7450-56. doi: 10.3748/wjg.v11.i47.7450
  10. Soni H, Shah A, Paul A, Patel G. Anti-ulcer activity of herbo-mineral formulation (Asecure capsule) against experimentally induced acute and chronic gastric ulcers in rats. Int J Pharmacol Clin Sci. 2014 Sep;3(Is):61-67. https://www.ijpcs.net/sites/default/files/IJPCS_3_3_04.pdf
Address for correspondence:
644043, The Russian Federation,
Omsk, Lenin Str., 12,
Omsk State Medical University,
Department of Faculty Surgery, Urology.
Tel.: +7 3812 35-91-30,
e-mail: nikitin-1966@inbox.ru,
Vyacheslav N. Nikitin
Information about the authors:
Nikitin Vyacheslav N., PhD, Associate Professor of the Department of Faculty Surgery, Urology, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0002-7250-9266
Poluektov Vladimir L., MD, Professor, Head of the Department of Faculty Surgery, Urology, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0002-9395-5521
Khramykh Tatyana P., MD, Professor, Head of the Department of Topographic Anatomy and Operative Surgery, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0002-5508-6679
Barskaya Lyubov O., Assistant of the Department of Topographic Anatomy and Operative Surgery, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0002-0460-4296
Zablotskaia Elena A., Morbid Anatomist, Omsk Regional Clinical Medical Sanitary Unit 9, Omsk, Russian Federation.
https://orcid.org/0000-0002-4937-7699
Putintsev Vladislav A., 5-Year Medical Student, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0002-6396-8727
Efanov Vladimir P., 5-Year Medical Student, Omsk State Medical University, Omsk, Russian Federation.
https://orcid.org/0000-0001-6512-9831
Contacts | ©Vitebsk State Medical University, 2007