Year 2019 Vol. 27 No 1




Institute of Chemical Biology and Fundamental Medicine1,
Medical Center Avicenna 2,
Institute of Molecular Pathology and Pathomorphology, Federal Research Center for Fundamental and Translational Medicine3,
The Russian Federation

Objective. To study the possibility of complications development after the injection of the autologous multipotent mesenchymal stromal cells of the bone marrow origin (MMSC) directly in the intact liver of rats.
Methods. The changes of the rat liver after the direct injection of MMSC with a transfected GFP-gene and additionally stained cellular membranes by Vybrant CM-Dil were investigated by the methods of light microscopy with luminescence application.
Results. After the MMSC injections, the extensive congestions, depot of the injected cells in the liver were absent in all animals even after a week. In some cases, up to 1/3 observations, the aseptic inflammation manifested by the dilation and hyperemia of sinusoids, an increase of neutrophil number in the sinusoids, necroses of parenchyma and lympho-histiocytic infiltrates were developed in the organ. Long thin layers of the connective tissue extending in the vessel region and containing a large number of siderophages appeared in the liver during the 3rd week. By the 4th week the specified liver morphological changes have normalized. Throughout the experiment there was no any differentiation of injected MMSC to the liver cells.
Conclusions. The main causes of the revealed changes are the damage of the liver parenchyma at the cell suspension injection under pressure and also direct presence of MMSC and their detritus. At the syringe under pressure the MMSC suspension moves apart and lacerates the liver parenchyma along the vessels. Injected MMSC, some of which quickly perishes due to a dramatic change in living conditions, and also the detritus which is present together with them, immediately cause migration of macrophages to the site of injection and actually are the reason of aseptic inflammatory process. MMSC are quickly eliminated from the injection site in the liver by macrophages and also by means of vascular and lymphatic beds whereas the proceeding inflammatory reaction damages the liver cells and serves as the cause of the revealed changes.

Keywords: multipotent mesenchymal stromal cells, liver, inflammation, necrosis, animals, experiment
p. 5-15 of the original issue
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Address for correspondence:
630090, The Russian Federation,
Novosibirsk, Ac. Lavrentyev Ave., 8,
Institute of Chemical Biology
and Fundamental Medicine,
Center of Innovative Medical Technologies.
Tel.: 8-913-753-0767,
Igor V. Maiborodin
Information about the authors:
Maiborodin Igor V., MD, Professor, Chief Researcher of the Stem Cell Laboratory, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russian Federation.
Figurenko Nikolay F., PhD, Applicant for Doctors Degree of the Stem Cell Laboratory, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russian Federation.
Elovskiy Aleksandr A., Anesthesiologist-Resuscitator-Transfusiologist of the Anesthesiology and Resuscitation Unit, Medical Center Avicenna, Novosibirsk, Russian Federation.
Mikheeva Tatiana V., PhD, Applicant for Doctors Degree of the Stem Cell Laboratory, Institute of Chemical Bio logy and Fundamental Medicine, Novosibirsk, Russian Federation.
Maslov Roman V., PhD, Applicant for Doctors Degree of the Stem Cell Laboratory, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russian Federation.
Maiborodina Vitalina I., MD, Leading Researcher of the Laboratory of Ultrastructural Basis of Pathology, Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russian Federation.
Shevela Andrey I., MD, Professor, Head of the Unit Center of innovative medical technologies, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russian Federation.
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