Novosti
Khirurgii
This journal is
indexed in Scopus









Year 2021 Vol. 29 No 4

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2021.4.401   |  

I.V. MAIBORODIN 1, 2, A.A. SHEVELA 2, S.V. MARCHUKOV 2, V.V. MOROZOV 2, V.A. MATVEEVA 2, V.I. MAIBORODINA 1, A.M NOVIKOV 3, YU.V. TORNUEV 1, B.V. CHURIN 1, A.I. SHEVELA 2

PROLONGATION OF CLEANSING DAMAGED TISSUES FROM DETRITUS USING EXOSOMES OF MULTIPOTENT STROMAL CELLS

Federal Research Center of Fundamental and Translational Medicine of the Ministry of Science and Higher Education of the Russian Federation, Institute of Molecular Pathology and Pathomorphology 1,
Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Branch 2,
Research Institute of Clinical and Experimental Lymphology 3, Novosibirsk,
The Russian Federation

Objective. To study the effect of exosomes of multipotent mesenchymal stromal cells (EMSCs) on soft tissues damaged during implantation of a metal screw into the bone.
Methods. A defect (2 mm in diameter and 4 mm in depth) was created in the tibial proximal condyles of outbred rabbits. Metal screws were implanted into the defect by preliminary injection of saline (control, n=9 animals) or 19.2 μg of EMSCs per limb (experiment, n=10 rabbits). After 3, 7 and 10 days following the operation, the animals were taken out from the experiment; histological sections of soft tissues from the condyle surface, stained by hematoxylin and eosin were studied using light microscopy.
Results. The use of water cooling in the process of introducing the metal implant into the tibial proximal condyle does not lead to complete removal of small bone fragments, which are subsequently either eliminated outward with wound discharge, or are destroyed and are subjected to lysis by macrophages. As a result of the EMSC effect on soft tissues near the site of damage, the activity of the postoperative inflammation reduces, leads to a slowdown in the resorption of hemorrhages, the elimination of fibrin clots, detritus and small bone fragments. Even on the 10th day after using EMSCs in the postoperative wound a structureless detritus with a small number of infiltrating cells was present, as well as a significant number of multinucleated macrophages with fused cytoplasm, non-viable lysed striated muscle symplasts and bone fragments with a low degree of degradation.
Conclusion. Suppression of inflammation by EMSCs delays the clearance of the postoperative wound, promotes the prolongation of the repair process and the attachment of the granulomatous component to the inflammation. The using EMSCs in the process of intraosseous implantation may be recommended only to control the activity of the inflammatory process and only after maximum preliminary cleansing of the postoperative wound from detritus, including non-viable muscle tissue and bone fragments.

Keywords: damaged tissues, exosomes of multipotent mesenchymal stromal cells, regeneration, inflammation, macrophages
p. 401-411 of the original issue
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Address for correspondence:
630090, Russian Federation,
Novosibirsk, Ak. Lavrentyev Avenue, 8,
Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences,
Siberian Branch Center of New Medical Technologies
tel.: +7-913-753-0767
e-mail: imai@mail.ru
Maiborodin Igor V.
Information about the authors:
Maiborodin Igor V., MD, Professor, Chief Researcher of the Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal Research Center for Fundamental and Translational Medicine of the Ministry of Science and Higher Education of the Russian Federation, Chief Researcher of the Laboratory of Health Management Technologies, Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
https://orcid.org/0000-0002-8182-5084
Shevela Aleksandr A., Ph.D, Doctoral Student, the Laboratory of Health Management Technologies, Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
http://orcid.org/0000-0001-9235-9384
Marchukov Sergey V., Ph.D, Doctoral Student, the Laboratory of Health Management Technologies, Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
https://orcid.org/0000-0002-7381-5820
Morozov Vitaly V., MD, Professor, Head of the Laboratory of Health Management Technologies, Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
https://orcid.org/0000-0002-9810-5593
Matveeva Vera A., PhD, Senior Researcher of the Laboratory of Health Management Technologies, Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
https://orcid.org/0000-0002-8706-4853
Maiborodina Vitalina I., MD, Leading Researcher, the Laboratory of Ultrastructural Basis of Pathology, Institute of Molecular Pathology and Pathomorphology, Federal ResearchCenter for Fundamental and Translational Medicine of the Ministry of Science and Higher Education of the Russian Federation, Novosibirsk, Russian Federation.
http://orcid.org/0000-0002-5169-6373
Novikov Alexey M., Junior Researcher, the Laboratory of Cell Technology, Institute of Clinical and Experimental Lymphology, the Branch of the Federal State Budgetary Scientific Institution Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, The Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
http://orcid.org/0000-0003-1371-7492
Tornuev Yury V., MD (Biol), Professor, Chief Researcher, the Laboratory of General Pathology and Pathomorphology, Institute of Molecular Pathology and Pathomorphology, Federal State Budgetary Scientific Institution Federal Research Center for Fundamental and Translational Medicine of the Ministry of Science and Higher Education of the Russian Federation, Novosibirsk, Russian Federation.
http://orcid.org/0000-0001-8629-8909
Churin Boris V., MD, Professor, Chief Researcher, the Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal State Budgetary Scientific Institution Federal Research Center for Fundamental and Translational Medicine of the Ministry of Science and Higher Education of the Russian Federation, Novosibirsk, Russian Federation.
http://orcid.org/0000-0001-9742-6152
Shevela Andrey I., MD, Professor, Head of Department Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation.
http://orcid.org/0000-0002-3164-9377
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