Novosti
Khirurgii
This journal is
indexed in Scopus









Year 2022 Vol. 30 No 1

GENERAL & SPECIAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2022.1.38   |  

R.I. DOVNAR 1, A.YU. VASILKOV 2, T.M. SAKALOVA 1, I.E. BUTENKO 2, S.M. SMOTRYN 1, N.N. IASKEVICH 1

ANTIBACTERIAL ACTION OF SILVER NANOPARTICLES

Grodno State Medical University 1, Grodno,
The Republic of Belarus,
A.N.Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences 2, Moscow,
The Russian Federation

Objective. To determine the minimum inhibitory concentration of Ag nanoparticles in relation to clinical pathogenic strains of microorganisms.
Methods. The minimum inhibitory concentration of Ag nanoparticles, obtained by metal vapor synthesis was studied on six strains of pathogenic bacteria, including representatives of gram-positive and gram-negative groups. The microbiological analyzer Vitek 2 Compact was used to identify each strain and to determine the antibiogram. The metal nanoparticles used in the study were synthesized by the method of metalvapor synthesis. Ag nanoparticles were studied by transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) methods. Determination of the minimum inhibitory concentration was performed by the method of serial dilution using sterile 96-well plates with using the tests of positive and negative control. The concentration of microorganisms was controlled by the turbidity standard.
Results. All pathogenic strains of bacteria used in the study were characterized by pronounced polyantibiotic resistance, and the percentage of antibiotics against which the strain was resistant ranged from 12.5 to 93.3%. The minimum inhibitory concentration of silver nanoparticles ranged from 7.81 to 31.25 μg/ml, depending on the type of microorganism. Gram-positive microorganisms, in contrast to gram-negative ones, were characterized by lower values of the minimum inhibitory concentration. The data of transmission electron and X-ray photoelectron spectroscopy showed that the size of the studied nanoparticles is in the range of 2-15 nm.
Conclusion. Silver nanoparticles (2-15 nm in size) have antimicrobial action against clinically significant, polyantibiotic-resistant strains of microorganisms. The minimum inhibitory concentration of silver nanoparticles, depending on the strain of the microorganism, varies from 7.81 to 31.25 μg/ml. Silver nanoparticles have an inhibitory impact on microorganisms and to a greater extent inhibit the growth of gram-positive versus gram-negative. The obtained materials based on silver nanoparticles represent an effective alternative to the currently used antibacterial drugs.

Keywords: silver, metal nanoparticles, anti-bacterial agents, drug resistance, microbial sensitivity tests, nanotechnology, green chemistry technology
p. 38-45 of the original issue
References
  1. Bennett JW, Chung KT. Alexander Fleming and the discovery of penicillin. Adv Appl Microbiol. 2001;49:163-84. doi: 10.1016/s0065-2164(01)49013-7
  2. Lobanovska M, Pilla G. Penicillins discovery and antibiotic resistance: lessons for the future? Yale J Biol Med. 2017 Mar 29;90(1):135-45. eCollection 2017 Mar. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369031/
  3. Ahmad M, Khan AU. Global economic impact of antibiotic resistance: a review. J Glob Antimicrob Resist. 2019 Dec;19:313-16. doi: 10.1016/j.jgar.2019.05.024
  4. Fedotcheva TA, Olenin AYU, Starostin KM, Lisichkin GV, Banin VV, Shimanovskij NL Prospects for using gold, silver, and iron oxide nanoparticles for increasing the efficacy of chemotherapy. Him-Farm Zhurn. 2015;49(4):11-22. doi: 10.1007/s11094-015-1260-6 (In Russ.)
  5. Gherasim O, Puiu RA, Bircă AC, Burdu?el AC, Grumezescu AM. An Updated Review on Silver Nanoparticles in Biomedicine. Nanomaterials (Basel). 2020 Nov 23;10(11):2318. doi: 10.3390/nano10112318
  6. Blagitko EM, Burmistrov VA, Kolesnikov AP, Mihajlov JuI, Rodionov PP. Serebro V Medicine. Novosibirsk, RF: Nauka-Centr; 2004. 254 p. (In Russ.)
  7. Abd-Elsalam KA, Alghuthaymi MA, Shami A, Rubina MS, Abramchuk SS, Shtykova EV, Vasilkov AYu. Copper-chitosan nanocomposite hydrogels against aflatoxigenic Aspergillus flavus from dairy cattle feed. J Fungi (Basel). 2020 Jul 21;6(3):112. doi: 10.3390/jof6030112
  8. Vasilkov AY, Dovnar RI, Smotryn SM, Iaskevich NN, Naumkin AV. Plasmon resonance of silver nanoparticles as a method of increasing their antibacterial action. Antibiotics (Basel). 2018 Aug 22;7(3):80. doi: 10.3390/antibiotics7030080
  9. Beamson G, Briggs D. High resolution XPS of organic polymers: The scienta ESCA300 database. Chichester, GB: Wiley; 1992. 306 p.
  10. Naumkin AV, Kraut-Vass A, Gaarenstroom SW, Powell CJ. NIST X-ray photoelectron spectroscopy database, NIST standard reference database number 20. Gaithersburg, USA: National Institute of Standards and Technology; 2000. doi: 10.18434/T4T88K
Address for correspondence:
230009, Belarus,
Grodno, Gorky Str., 80,
Grodno State Medical University,
the Department of Surgical Diseases No2,
tel. +375 297 868643,
e-mail: dr_ruslan@mail.ru,
Dovnar Ruslan I.
Information about the authors:
Dovnar Ruslan I., PhD, Associate Professor, Associate Professor of the Department of Surgical Diseases No2, Grodno State Medical University, Grodno, Republic of Belarus.
https://orcid.org/0000-0003-3462-1465
Vasilkov Alexander Yu., l PhD (Chem), Associate Professor, Leading Researcher of the Laboratory of Hybrid Metal-Containing Materials of A.N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences, Moscow, Russian Federation.
https://orcid.org/0000-0001-8225-647X
Sakalova Tatsiana M., PhD, Associate Professor, Associate Professor of the Department of Microbiology, Virology and Immunology, Grodno State Medical University, Grodno, Republic of Belarus.
https://orcid.org/0000-0002-4075-4515
Butenko Ivan E., Senior Engineer of the Laboratory of Hybrid Metal-Containing Materials of A.N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences, Moscow, Russian Federation.
https://orcid.org/0000-0002-1741-6155
Smotryn Siarhei M., MD, Professor, Professor of the Department of Surgical Diseases No2, Grodno State Medical University, Grodno, Republic of Belarus.
https://orcid.org/0000-0002-3944-1124
Iaskevich Nikolai N., MD, Professor, Professor of the Department of Surgical Diseases No1, Grodno State Medical University, Grodno, Republic of Belarus.
https://orcid.org/0000-0002-2954-0452
Contacts | ©Vitebsk State Medical University, 2007