Novosti
Khirurgii
This journal is
indexed in Scopus









Year 2015 Vol. 23 No 3

GENERAL & SPECIAL SURGERY

DOI: http://dx.doi.org/10.18484/2305-0047.2015.3.302   |  

S.A. SUSHKOU, I.V. SAMSONOVA, M.M. GALISHEVICH

CD34 EXPRESSION PATOGENETIC VALUE IN LOWER LIMB VARICOSE VEINS

EE "Vitebsk State Medical University",
The Republic of Belarus

Objective. To study the CD34 expression in the leg vein wall of patients with the lower limb varicose veins.
Methods. The study included 18 patients who were subjected to the resection of the posterior tibial veins together with phlebectomy because of the diagnosed deep venous valvular insufficiency. For morphological examination the resected specimens of the posterior tibial veins and the distal fragment of the vena saphena magna taken by phlebectomy were used. The samples of the corresponding veins without any pathology obtained from died patients not suffering from the lower limb varicose veins were used for control.
Serial sections were stained with routine histological methods and immunohystochemically using monoclonal antibodies against CD34. The degree of CD34 expression in the specimens was evaluated by light microscopy.
Results. In the posterior tibial vein wall of the patients with lower limb varicosity and deep venous valvular insufficiency, the degree of its expression in the endothelium comparing was different from the control group. The total area of expression was 0,00850,0004 μm2, in control samples it was significantly less 0,0040,0003 μm2; (p<0,05). In the vena saphena magna the specimens taken from the patients with varicose veins, CD34 expression area was also significantly higher than in the control group 0,01160,0009 μm2 (in control group 0,0050,0004 μm2; p<0,05). Comparison of CD34 expression in the specimens of the vena saphena magna and posterior tibial veins taken from the patients with lower limb varicose veins showed reliably higher parameters in the vena saphena magna (p<0,05).
Conclusion. At lower limbs superficial varicose veins the unidirectional changes in the endothelium of the superficial and deep leg veins are developing; its proved by change in the CD34 expression on the endothelial cell surface.

Keywords: primary varicose veins, endothelium, vein wall, immunohistochemistry, CD34 expression, light microscopy, phlebectomy
p. 302-308 of the original issue
References
  1. Bergan J. Molecular mechansms in chronic venous insufficiency. Ann Vasc Surg 2007 May;21(3):260-66.
  2. Perrin M, Ramelet AA. Pharmacological treatment of primary chronic venous disease: rationale, results and unanswered questions. Eur J Vasc Endovasc Surg 2011 Jan; 41(1):117-25.
  3. Pascarella L, Schönbein GW, Bergan JJ. Microcirculation and venous ulcers: a review. Ann Vasc Surg 2005 Nov; 19(6):921-27.
  4. Nicolaides AN. Chronic venous disease and the leukocyte-endothelium interaction: from symptoms to ulceration. Angiology 2005 Sep-Oct; 56(Suppl 1):S11-19.
  5. Raffetto JD, Khalil RA. Mechanisms of varicose vein formation: valve dysfunction and wall dilation. Phlebology 2008; 23(2):85-98.
  6. Bergan JJ, Pascarella L, Schmid-Schonbein G. Pathogenesis of primary chronic venous disease: insights from animal models of venous hypertension. J Vase Surg 2008 Jan; 47(1):183-92.
  7. Ojdana D, Safiejko K, Lipska A, Sacha P, Wieczorek P, Radziwon P, Dadan J, Tryniszewska E. The inflammatory reaction during chronic venous disease of lower limbs. Folia Histochem Cytobiol 2009; 47(2):185-89.
  8. Raffetto JD. Dermal pathology, cellular biology, and inflammation in chronic venous disease. Thromb Res 2009; 123(Suppl 4):S6671.
  9. Liu YC, Margolis DJ, Isseroff RR. Does inflammation have a role in the pathogenesis of venous ulcers? A critical review of the evidence. J Invest Dermatol. 2011 Apr;131(4):818-27. doi: 10.1038/jid.2010.428.
  10. Lim CS, Davies AH. Pathogenesis of primary varicose veins. Br J Surg 2009 Nov; 96(11):1231-42.
  11. Pascarella L, Penn A, Schmid-Schönbein GW. Venous hypertension and the inflammatory cascade: major manifestations and trigger mechanisms. Angiology 2005 Sep-Oct; 56(Suppl 1):S310.
  12. Simovart HE, Aunapuu M, Lieberg J, Roosaar P, Arend A. Age-related changes in apoptosis and expressions of intercellular adhesion molecule-1 and vascular endothelial growth factor receptor type 2 in the wall of varicose veins. Int Angiol. 2010 Dec;29(6):507-13.
  13. Erguzel N, Yetkin E, Erdem G, Erdil N, Yetkin G, Heper G, Celik T, Senen K. Plasminogen activator inhibitor-1 levels in patients with primary varicose vein. Int Angiol. 2012 Apr;31(2):176-80.
  14. Zhukov BN, Katorkin SE, Kravtsov PF. Optimizatsiia diagnosticheskoi programmy i pokazanii k khirurgicheskoi korrektsii nedostatochnosti klapannogo apparata glubokikh ven u bol'nykh varikoznoi bolezn'iu nizhnikh konechnostei [Optimization of the diagnostic program and the indications for surgical correction of deep vein valve failure in patients with varicose veins of the lower extremities]. Novosti Khirurgii. 2010;8(1):46-51.
  15. Sushkou SA. Korregiruiushchie vmeshatel'stva na zadnikh bol'shebertsovykh venakh pri varikoznoi bolezni [Corregiall interventions on the posterior tibial veins with varicose veins]. Novosti Khirurgii 2008;16(3):42-52.
  16. Kachlík D, Stingl J, Sosna B, Straka Z, Lametschwandtner A, Minnich B, Fára P. Morphological features of vasa vasorum in pathologically changed human great saphenous vein and its tributaries. Vasa. 2008 May;37(2):127-36. doi: 10.1024/0301-1526.37.2.127.
  17. Aunapuu M, Arend A. Histopathological changes and expression of adhesion molecules and laminin in varicose veins. Vasa. 2005;(34)3:170-75.
Address for correspondence:
210023, Respublika Belarus,
g. Vitebsk, pr. Frunze, d. 27,
UO "Vitebskiy gosudarstvennyiy meditsinskiy universitet",
kafedra obschey khirurgii,
e-mail: sergery@nm.ru,
Sushkov Sergey Albertovich
Information about the authors:
Sushkov S.A.PhD, an associate professor, Vice-rector (Science) of EE "Vitebsk State Medical University".
Samsonova I.V. PhD, an associate professor, a head of the pathological anatomy chair of EE "Vitebsk State Medical University".
Galishevich M.M. The 6th-year student of the medical faculty of EE "Vitebsk State Medical University".
Contacts | ©Vitebsk State Medical University, 2007