Year 2022 Vol. 30 No 2

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

V.I. LUPALTSOV, S.S. KITCHENKO, I.V. SOROKINA, O.V. KALUZHYNA

LOCAL TRANSPLANTATION OF STROMAL CELLS OF ADIPOSE TISSUE IN THE COMPARATIVE ASSESSMENT OF THE HEALING PARAMETERS OF AN EXPERIMENTAL VENOUS TROPHIC ULCER

Kharkiv National Medical University, Kharkiv
Ukraine

Objective. To study the effectiveness of adipose tissue stromal cells in the restoration of the epidermal layer of an experimental venous trophic ulcer.
Methods. The formation of an experimental trophic ulcer was performed in 14 rabbits of the Chinchilla line. 7 rabbits (main group) in complex treatment underwent autotransplantation of multipotent mesenchymal stromal cells (MMSC) isolated from adipose tissue according to the method of Zuk et al.; 7 rabbits (control group) in local treatment used daily dressings with treatment of ulcers with dioxidine solution and subsequent application of hydrophilic ointment («Levomecol»). In both groups on the 1st, 7th, 14th, and 21st days, morphological (demarcation shaft, relative volume of leukocyte-necrotic zone and granulation tissue) and immunohistochemical (determination of the optical density of immunofluorescence of collagen types I and II, quantitative counting of immune cells producing IL-6 and TNFα) parameters.
Results. On the 7th day in the main group, the indicators of leukocyte-necrotic and leukocyte-demarcation shafts were 49.21 ± 2.0% and 18.03 ± 1.6%, respectively, in the comparison group – 51.09 ± 5.8%. Granulation tissue was 32.55 ± 2.4% in the main group, 29.73 ± 2.9% in the comparison group. The optical luminescence density of type III collagen exceeded that of type I collagen and was 0.069 ± 0.001 conventional units of collagen luminescence in the main group and 0.036 ± 0.001 conventional units of collagen luminescence - in the control group. On the 21st day in the main group there was a decrease in necrotic lesions, granulation tissue contained a large number of microvessels, fibroplastic cells, macrophages, lymphocytes, a large number of giant multinucleated cells. The number of cells expressing interleukin-6 and tumor necrosis factor-α decreased significantly, there was a predominance of young interstitial collagen type III.
Conclusion. The use of cellular therapy by autotransplantation of MMSCs isolated from adipose tissue in the local treatment of experimental «venous» trophic ulcer, accelerates reparative processes and allows to achieve its healing.

Keywords: multipotent mesenchymal stromal cells, «venous» trophic ulcer, cell therapy, chronic venous insufficiency, experiment, morphological study, immunohistochemical studies
p. 135-143 of the original issue
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Address for correspondence:
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Lupaltsov Vladimir I.
Information about the authors:
Lupaltsov Vladimir I., MD, Professor, Corresponding Member of NAMS of Ukraine, Head of the Surgery Department No3, Kharkiv National Medical University, Kharkiv, Ukraine.
http://orcid.org/0000-0003-2121-529X
Kitchenko Sergey S., Assistant of the Surgery Department No3, Kharkiv National Medical University, Kharkiv, Ukraine.
http://orcid.org/0000-0001-6330-6187
Sorokina Irina V., MD, Professor, Acting Head of the Pathologic Anatomy Department, Kharkiv National Medical University, Kharkiv, Ukraine.
http://orcid.org/0000-0002-5945-2605
Kaluzhyna Oksana V., PhD, Associate Professor of the Pathologic Anatomy Department, Kharkiv National Medical University, Kharkiv, Ukraine.
https://orcid.org/0000-0002-4181-9704
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