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Khirurgii

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Year 2016 Vol. 24 No 6

GENERAL & SPECIAL SURGERY

V.K. OKULICH

ABZYME ACTIVITY OF IMMUNOGLOBULINS IN SURGICAL INFECTION

EE "Vitebsk State Medical University",
Vitebsk,
The Republic of Belarus

Objectives. To determine the role of proteolytic, DNAse, esculinase and urease activity of abzymes in clinical and laboratory picture and to assess the capacity to destroy exopolymeric matrix of biofilms in patients with surgical infection.
Methods. All the patients tested for abzyme activity were divided into the following groups: a group of persons with various chronic inflammatory processes; with spreading inflammatory processes; persons with local acute inflammatory processes, pyoinflammatory disease of the maxillofacial area, chronic osteomyelitis, cholecystitis, adenomatous goiter, gastroduodenitis and severe form of pneumonia. Control group consisted of donors of blood transfusion station and patients without pyoinflammatory processes. Proteolytic activity of immunoglobulin G in reactions with chromogenic substrates: benzoyl-arginine-p-nitroanilide (BAPNA), lysine-p-nitroanilide (LPA), arginine-proline-p-nitroanilide (APNA) and acetyl-aspartate-p-nitroanilide (AANA) was determined in patients with surgical infection and healthy donors.
Results. It was found out that the rate of BAPNA-amidase abzyme activity in patients with surgical infection was reliably higher, LPA- and APNA-amidase rate didn’t differ and AANA-amidase rate was significantly lower than in donors. It was revealed that immunoglobulins derived from patients with surgical infection have the ability to destroy the exopolymeric matrix of the biofilm. Presence of reliably elevated levels of DNAse and esculinase activity of IgG in surgical patients with pyo-inflammatory processes in comparison with patients without suppurative complications was determined. Positive reliable correlation between type of causative agent and DNAse activity of IgG was revealed. The elevated levels of urease activity of IgG, derived from patients with gastroduodenitis, associated with H. pylori in comparison with patients without H. pylori infection was shown.
Conclusion. Obtained data concerning catalytic activity of IgG in patients with infectious process may be used for development of new diagnostic criteria and methods for prognosis of pyoinflammatory diseases.

Keywords: surgical infection, catalytic antibodies, abzymes, biofilm, proteolytic activity, diagnostic criteria, pyoinflammatory diseases
p. 568-578 of the original issue
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Address for correspondence:
210023, Republic of Belarus,
Vitebsk, pr. Frunze, 27, EE "Vitebsk
State Medical University",
department of clinical microbiology,
Tel.: +375 212 06.12.37
E-mail: vokul@mail.ru
Okulich Vitaly Konstantinovich
Information about the authors:
Okulich V.K. PhD, Ass. Professor of department of clinical microbiology, EE "Vitebsk State Medical University".
Contacts | ©Vitebsk State Medical University, 2007-2023