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Year 2016 Vol. 24 No 1

GENERAL & SPECIAL SURGERY

N.N. YAROTSKAYA, I.V. SAMSONOVA, V.A. KOSINETS

THE INFLUENCE OF METABOLIC PREPARATIONS ON INTENSITY OF CYTOCHROME C EXPRESSION AT EXPERIMENTAL GENERALIZED PURULENT PERITONITIS

EE ''Vitebsk State Medical University'',
The Republic of Belarus

Objectives. To study the influence of metabolic preparations "Citoflavin" and "Neoton" on cytochrome c expression of apoptotic markers in the rabbit liver at experimental generalized purulent peritonitis.
Methods. The immunohistochemical analysis of cytochrome c expression intensity in the liver specimens of rabbits (n=55) was carried out at experimental generalized purulent peritonitis. Tissue samples were stained using antibodies to cytochrome c ("Sigma", USA). The analysis of serial microphotos was performed by the digital analytical system (microscope Leica DM 2000, Leica Application Suite 3.6.0 program.). All morphometric parameters were manually measured using ImageJ 1.45S software with histological quantification assessed intensity of staining agents.
Results. Liver sections revealed its presence in cytoplasm of hepatocytes both at purulent peritonitis and in samples of intact group as light to dark brown staining of different intensity. At the same time in the early stages of peritonitis initiation the level of cytochrome c expression as a apoptotic signal significantly decreases due to the damage of membrane structures of hepatocytes, accompanied by the modification activity of many intracellular enzymes, inhibition of antitoxic liver function, violation of synthetic processes, dissociation in respiring tissues and oxidative phosphorylation, reduction in ATP synthesis, development of hypoxia. The preparation "Citoflavin" (containing amber acid) in comparison to the preparation "Neoton" (containing creatinephosphate) on the 5th day of the postoperative period leads to gradual restoration of the cytochrome c level. It may be a result of a positive influence of the preparation on energy processes in a cell, thus increasing resistance to intoxication.
Conclusion. The cytochrome c expression is a marker for the early diagnosis at experimental generalized purulent peritonitis. Reduction of its intensity is correlated with the massive hepatocyte apoptosis due to the severe intoxication.

Keywords: experimental generalized purulent peritonitis, cytochrom c, Citoflavin, Neoton, amber acid, creatinephosphate, resistance to intoxication
p. 62-69 of the original issue
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Address for correspondence:
210022, Republic of Belarus
Vitebsk, pr-t Frunze, d. 27, kor. 3,
UO ''Vitebskiy gosudarstvennyiy
meditsinskiy universitet '',
nauchno-issledovatelskaya laboratoriya,
tel. mob: 375 29 714-18-76,
e-mail: yardip@yandex.ru,
Yarotskaya Natalya Nikolaevna
Information about the authors:
Yarotskaya N.N. A researcher of scientific research laboratory of EE ''Vitebsk State Medical University ''.
Samsonova I.V. PhD, an associate professor, a head of the pathologic anatomy chair of EE ''Vitebsk State Medical University ''.
Kosinets V.A. MD, professor of the hospital surgery chair with the courses of urology and pediatric surgery of EE ''Vitebsk State Medical University''.
References
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