Novosti
|
This journal is indexed in Scopus |
|---|
Year 2012 Vol. 20 No 4
EXPERIMENTAL SURGERY
I.S. IVANOV, S.V. IVANOV, G.N. GORYAINOVA, A.V. IVANOV, D.V. TARABRIN
EXPERIMENTAL USING OF CELLULAR TECHNOLOGIES TO IMPROVE THE PROPERTIES OF CONNECTIVE TISSUE
Federal Budgetary State Establishment “Kursk State Medical University”
The Russian Federation
Objectives. To study the impact of the site of the ectogenous embryonic fibroblasts introduction in regard to endoprosthesis on the collagen metabolism dynamics.
Methods. The experiment was conducted on 150 mice. The sterile polytetrafluoroethylene prosthesis was placed above the aponeurosis. The animals were divided into 3 groups numbering 50 animals: the 1 group – without fibroblasts introduction; the 2 – a single introduction of fibroblasts on the 7 day; the 3 group – the fibroblasts were injected twice on the 7 and 10 days. Cultivated fibroblasts were obtained from the embryos of white mice. Ectogenous fibroblasts were injected in the paratransplant zone only on the side of prosthesis inverted to the skin and subcutaneous tissue. Animals were derived from the experiment on the 10, 30 and 60 days. The sections were stained with Sirius Red.
Results. It has been revealed that at early terms the introduction of fibroblasts and repetition factor of the introduction don’t influence the collagen types correlation depending on the spatial orientation of the prosthesis. Further a reliable increase of the collagen type I is registered. Introduction of the ectogenous fibroblasts accelerates the increase of collagen correlation of I and III types more in the case of repeated introduction. On the 30 and 60days of the experiment reliable differences in the collagen correlation of I and III types have been registered and the increase of the collagen type I content has been observed on the side of prosthesis inverted to the skin. Deposition of the ectogenous fibroblasts due to the structure of the polytetrafluoroethylene prosthesis material and their further participation in the collagen metabolism is considered to be a critical factor in the process of connective tissue capsule formation and prevalence of the collagen type I at the late terms of the experiment.
Conclusions. The application of the ectogenous fibroblasts has a reliable modifying effect on the dynamics of the collagen type I formation on the introduction side.
1. Egiev VN. Sravnitel'naia otsenka stepeni fiksatsii fibroblastov na sinteticheskikh endoprotezakh, ispol'zuemykh dlia plastiki defektov perednei briushnoi stenki [The comparative estimation of fibroblasts fixation degree on the synthetic endoprothesis used for the plastic of anterior abdominal wall defects]. Gerniologiia. 2006;(2):37–41.
2. Timoshin AD, Iurasov AV, Shestakov AL. Kontseptsiia khirurgicheskogo lecheniia posleoperatsionnykh gryzh perednei briushnoi stenki [The concept of surgical treatment of postoperative abdominal wall hernias]. Gerniologiia. 2004;(1):5–10.
3. Iarosh AL. Issledovanie biosovmestimosti khirurgicheskikh implantatov novogo pokoleniia dlia plastiki perednei briushnoi stenki Fundamental'nye issledovaniia. [The study of biocompatibility of new generation surgical implants for plasty of anterior abdominal wall]. Fundamental Issledovaniia. 2011;(10 Ч. 1):186–89.
4. Gananadha S, Samra JS, Smith GS, Smith RC, Leibman S, Hugh TJ. Hugh Laparoscopic ePTFE mesh repair of incisional and ventral hernias. ANZ Journal of Surgery. 2008 Oct;78(10):907–13.
5. Il'in DA. Model' vozdeistviia polimernogo materiala na kul'turu fibroblastov Estestvoznanie i gumanizm. [Impact model of polymeric material on fibroblasts culture]. Estestvoznanie i Gumanizm. 2006;3(3):91–97.
6. Nikitin VN. Vozrastnaia i evoliutsionnaia biokhimiia kollagenovykh struktur [Developmental and evolutionary biochemistry of collagen structures]. Kiev, SSSR: Naukova dumka, 1977. 297 p.
7. Junge K, Klinge U, Rosch R, Mertens PR., Kirch J, Klosterhalfen B, Lynen P, Schumpelick V. Decreased collagen type I/III ratio in patients with recurring hernia after implantation of alloplastic prostheses. Langenbecks Arch Surg. 2004 Feb;389(1):17–22.
8. Meyer AL, Berger E, Monteiro Jr O, Alonso PA, Stavale JN, Goncalves MP. Quantitative and qualitative analysis of collagen types in the fascia transversalis of inguinal hernia patients. Arq Gastroenterol. 2007 Jul-Sep;44(3):2303–34.
9. Rosch R, Klinge U, Si Z, Junge K, Klosterhalfen B, Schumpelick V. A role for the collagen I/III and MMP-1/-13 genes in primary inguinal hernia? BMC Med Genet. 2002;3:2.
10. Broderick G, McIntyre J, Noury M, Strom HM, Psoinos C, Christakas A, Billiar K, Hurwitz ZM, Lalikos JF, Ignotz RA, Dunn RM. Dermal collagen matrices for ventral hernia repair: comparative analysis in a rat model. Hernia. 2012 Jun;16(3):333–43.
11. Hebda PA, Dohar JE. Transplanted fetal fibroblasts: survival and distribution over time in normal adult dermis compared with autogenic, allogenic, and xenogenic adult fibroblasts. Otolaryngol Head Neck Surg. 1999;121(3):245–51
305000, Rossiyskaya FederatsiyaKursk, Ul. K. Marksa, d. 3,GBOU VPO «Kurskiy gosudarstvennyiy meditsinskiy universitet»,kafedra hirurgicheskih bolezney №1
e-mail: ivanov.is@mail.ru,
Ivanov Ilya Sergeevich
Ivanov I.S., Candidate of Medical Sciences, Associate Professor of the Surgical Diseases Chair №1 of Federal State Budgetary State Establishment “Kursk State Medical University”
Ivanov S.V., Doctor of Medical Sciences, Professor, Head Of The Surgical Diseases chair №1 of Federal State Budgetary State Establishment “Kursk State Medical University”
Goryainova G.N., Candidate of Medical Sciences, Associate Professor of the Pathologic Anatomy Chair of Federal State Budgetary State Establishment “Kursk State Medical University”
Ivanov A.V., Doctor of Medical Sciences, Professor, Head of Histology, Embryology and Cytology Chair of Federal State Budgetary State Establishment “Kursk State Medical University”
Tarabrin D.V., Resident of the Surgical Diseases Chair №1 of Federal State Budgetary State Establishment “Kursk State Medical University”.
Article in the .pdf format
