Novosti
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This journal is indexed in Scopus |
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Year 2015 Vol. 23 No 4
SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY
B. S. SUKOVATYKH, A.Y. ORLOVA, E.B. ARTYUSHKOVA, M.Y. GORDOV, K.Y. VEDENEV
THE EFFECTIVENESS OF MONONUCLEAR FRACTION OF AUTOLOGOUS BONE MARROW IN TREATMENT OF EXPERIMENTAL CRITICAL LIMB ISCHEMIA
SBEE HPE "Kursk State Medical University"
The Russian Federation
Objectives. To develop an experimental model of critical limb ischemia for the further study of the effectiveness of the mononuclear fraction of autologous bone marrow in the treatment of critical limb ischemia.
Methods. The results of the application of the mononuclear fraction of autologous bone marrow in rats (Wistar) divided into four groups: intact, control, comparison and experimental have been analyzed. The intact group included 10 rats, the other groups – 30 animals in each. In all rats, except for the intact one, critical limb ischemia was created by excision of the main vessel, including the femoral artery, the popliteal artery and the initial segments of the leg arteries. Mononuclear fraction of autologous bone marrow from the femur contralateral limb of the rat in a volume of 200 μL was injected into the ischemic limb in the experimental group (every animal was given a 100 μL injection at two different injection sites: 1) directly below the inguinal ligament of the tissue in the area of the anatomical location of collaterals of the internal iliac artery; 2) intramuscularly in the gastrocnemius muscle of the anterior-lateral surface of the middle third of the tibia. In the control group the fraction was injected into the ischemic limb by infiltration at six different sites. In the control group the animals received no treatment. The treatment effectiveness was assessed by the dynamics of the microcirculation level by the 10th, 21st and 28th days by laser Doppler flowmetry.
Results. Assessment of the microcirculation in animals with experimental limb ischemia after treatment according to the original method in the experimental group with the compared group showed the increase in blood flow by the 10th days at the hip level by 1,5 folds, at leg – by 1,1 folds, by the 21st days at the level of the femur and tibia – by 1,4 folds and by the 28th days at the femur and tibia – by 1,2 folds.
Conclusion. The proposed method of treatment proven to be pathogenetically justified and effective.
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305041 Russian Federation,
Kursk, ul. K.Marksa, d. 3,
GBOU VPO "Kurskiy gosudarstvennyiy meditsinskiy universitet",
kafedra obschey khirurgii,
tel. office 8 4712 52-98-62,
e-mail: SukovatykhBS@kursksmu.net,
Sukovatykh Boris Semenovich
Sukovatykh B.S. MD, professor, a head of the general surgery chair of SBEE HPE "Kursk State Medical University".
Orlova A.Y. PhD, an assistant of the general surgery chair of SBEE HPE "Kursk State Medical University".
Artyushkova E.B. MD, a director of SRI of Ecological Medicine of SBEE HPE "Kursk State Medical University".
Gordov M.Y. A competitor of the general surgery chair of SBEE HPE "Kursk State Medical University".
Vedenev K.Y. A 3-year student of the medical faculty of SBEE HPE "Kursk State Medical University".
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