Novosti
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Year 2013 Vol. 21 No 6
EXPERIMENTAL SURGERY
I.A. SHVED, A.V. VOROBEY, T.E. VLADIMIRSKAYA, G.V. SHERSTYUK, O.P. SHORETS, S.V. ALEKSANDROV
THE PECULIARITIES OF LASER WOUNDS HEALING AFTER LIVER RESECTION BY HIGH-INTENSITY LASER RADIATION IN EXPERIMENT
SEE “Belarusian Medical Academy of Post-Graduate Education”,
The Republic of Belarus
Objectives. Studying the peculiarities of the laser wound healing after liver resection by high-intensity laser radiation (HILR) with different wavelength in experiment.
Methods. Experiments have been carried out on 64 rabbits. The resection of the portion of the liver was done by means of laser irradiation by a wavelength of 1064 nm, 1330 nm and 1440 nm. Samples of the blood and liver for hematologic and morphological studies were taken immediately and then on the 1st, 3rd, 7th, 14th, 30th days after operation.
Results. At all regimes the extensive zones of thermal liver injury and a secondary necrosis were formed, the zones of primary and secondary laser damages encapsulated, the remote damages developed perifocally. In an acute experiment more expressed macrophage reaction using the HILR regime at 1320 nm was registered. Inhibition of leukocyte reaction development was observed in animals using HILR at 1064 nm and 1440 nm. 14 days after the operation the output of purulent exudate on the surface of a fibrous capsule was observed in animals with the application of HILR at 1064 nm, substantial increase of segmented nuclei leukocytes number was revealed in the blood as well as the protein of a sharp phase and the erythrocyte sedimentation rate (ESR). By the 30th day of observation a full encapsulation with the constriction of the damage site and retention of non-resorbed necrosis areas of the liver tissue have been occurred in all animals.
Conclusions. It has been established that development of wound process, perifocal-distant changes and inflammatory reaction after liver resection with high-intensity laser irradiation depends on a used regime (1064 nm, 1320 nm and 1440 nm) in acute and chronic experiment. Healing of a liver wound occurs faster after radiation therapy by laser with the wavelength 1320 nm, causing more expressed macrophage reaction in a wound and increase of the total number of blood leukocytes in the early postoperative period.
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220013, Respublika Belarus', g. Minsk, ul. P.Brovki, d.3, k.3, GUO «Belorusskaia meditsinskaia akademiia poslediplomnogo obrazovaniia», nauchno-issledovatel'skaia laboratoriia,
e-mail: galinasher@mail.ru
Sherstyuk Galina Valentinovna
Shved I.A. MD, professor, a senior researcher of SRL SEE “Belarusian Academy of Post-graduate Education”.
Vorobey A.V. MD, professor, a head of the surgery chair of SEE “Belarusian Academy of Post-graduate Education”.
Vladimirskaya T.E. PhD, a leading researcher of SRL SEE “Belarusian Academy of Post-graduate Education”.
Sherstyuk G.V. PhD, a leading researcher of SRL SEE “Belarusian Academy of Post-graduate Education”.
Shorets O.A. A junior researcher of SRL SEE “Belarusian Academy of Post-graduate Education”.
Aleksandrov S.V. A lecturer of the surgery chair of SEE “Belarusian Academy of Post-graduate Education”.
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