Novosti
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This journal is indexed in Scopus |
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Year 2019 Vol. 27 No 5
TRAUNATOLOGY AND ORTHOPEDICS
P.G. KRAVCHUN 1, M.I. KOZHYN 1, F.S. LEONTIEVA 2, P.M. VORONTSOV 2, N.A. ASHUKINA 2, O.P. MARUSHCHAK 2, V.YU. DIELIEVSKA 1
REVEALING WEAK A AND B ANTIGENS IN PATIENTS WITH KNEE AND HIP JOINT ARTHROPLASTY
Kharkiv National Medical University 1,
Institute of Spine and Joint Pathology named after M. I. Sytenko 2, Kharkiv,
Ukraine
Objective. To analyze the methods of revealing weak A and B antigens on the erythrocytes in AB0 system.
Methods. Patients after knee and joint arthroplasty were examined on group-specific characteristics with revealing weak A and B antigens on the erythrocytes. Methods of absorption, agglutination, agglutination with complement and antiglobulin test were used.
Results. Antiglobulin test with the use of polyclonal serum as well as serum containing the only IgG allowed revealing weak A and B subgroups on erythrocytes at 37ºÑ. In some patients A and B antigens on erythrocytes were found while absorption with anti-A, anti-B polyclonal sera and also revealed in agglutination at 37ºÑ, but were not revealed while incubation at the room temperature. Agglutination test with the use of complement and IgG was also helpful in determining the weak antigens. Presence of IgG antibodies was revealed by the treatment of the serum with unithiol in antiglobulin test. Presence of complement and only IgG antibodies corresponding to the antigens led to the more expressed changes of erythrocytes as compared to the presence of both types of antibodies – IgM and IgG.
Appearance of hemolysis was associated with the increased sizes of erythrocytes and hypochromia. Presence of weak subgroups was mostly associated with hemolytic rather than agglutinating abilities of the patient’s serum, as well as with the presence of complement binding IgG antibodies.
Conclusions. Absorption, agglutination at 37ºÑ, antiglobulin test at 37ºC with serum both treated and non-treated with unithiol and agglutination with use of the complement helped to define weak antigens in AB0 system.
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61022, Ukraine,
Kharkiv, Nauky Ave.,4,
Kharkiv National Medical University,
Department of Internal Medicine ¹2,
of Clinical Immunology and Allergology
named after Academician L.T.Malaya.
Tel. +380688888201,
e-mail: valentinka_1987@ukr.net,
Dielievska Valentyna Yuriivna
Kravchun Pavlo G., MD, Professor, Head of the Department of Internal Medicine ¹2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
https://orcid.org/0000-0002-8285-6763
Kozhyn Mykhailo I., PhD, Associate Professor of the Department of Internal Medicine ¹2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
https://orcid.org/0000-0002-8359-8890
Leontyeva Frida S., PhD, Head of the Department of Laboratory Diagnostics and Immunology, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0001-9801-7908
Vorontsov Petr M., PhD, Head of the Department of Experimental Modeling and Transplantology, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-5758-7223
Ashukina Nataliya A., PhD, Head of the Morphology of Connective Tissue, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-0478-7440
Marushchak Oleksii P., Orthopedist and Traumatologist, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-5606-8763
Dielievska Valentyna Y., PhD, Assistant of the Department of Internal Medicine ¹2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
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