Novosti
Khirurgii
This journal is
indexed in Scopus









Year 2019 Vol. 27 No 5

TRAUNATOLOGY AND ORTHOPEDICS

DOI: https://dx.doi.org/10.18484/2305-0047.2019.5.530   |  

P.G. KRAVCHUN 1, M.I. KOZHYN 1, F.S. LEONTIEVA 2, P.M. VORONTSOV 2, N.A. ASHUKINA 2, O.P. MARUSHCHAK 2, V.YU. DIELIEVSKA 1

REVEALING WEAK A AND B ANTIGENS IN PATIENTS WITH KNEE AND HIP JOINT ARTHROPLASTY

Kharkiv National Medical University 1,
Institute of Spine and Joint Pathology named after M. I. Sytenko 2, Kharkiv,
Ukraine

Objective. To analyze the methods of revealing weak A and B antigens on the erythrocytes in AB0 system.
Methods. Patients after knee and joint arthroplasty were examined on group-specific characteristics with revealing weak A and B antigens on the erythrocytes. Methods of absorption, agglutination, agglutination with complement and antiglobulin test were used.
Results. Antiglobulin test with the use of polyclonal serum as well as serum containing the only IgG allowed revealing weak A and B subgroups on erythrocytes at 37º. In some patients A and B antigens on erythrocytes were found while absorption with anti-A, anti-B polyclonal sera and also revealed in agglutination at 37º, but were not revealed while incubation at the room temperature. Agglutination test with the use of complement and IgG was also helpful in determining the weak antigens. Presence of IgG antibodies was revealed by the treatment of the serum with unithiol in antiglobulin test. Presence of complement and only IgG antibodies corresponding to the antigens led to the more expressed changes of erythrocytes as compared to the presence of both types of antibodies IgM and IgG.
Appearance of hemolysis was associated with the increased sizes of erythrocytes and hypochromia. Presence of weak subgroups was mostly associated with hemolytic rather than agglutinating abilities of the patients serum, as well as with the presence of complement binding IgG antibodies.
Conclusions. Absorption, agglutination at 37º, antiglobulin test at 37ºC with serum both treated and non-treated with unithiol and agglutination with use of the complement helped to define weak antigens in AB0 system.

Keywords: antigen, blood, antiglobulin, complement, erythrocytes
p. 530-540 of the original issue
References
  1. Heier HE, Namork E, Calkovská Z, Sandin R, Kornstad L. Expression of A antigens on erythrocytes of weak blood group A subgroups. Vox Sang. 1994;66(3):231-36. doi: 10.1111/j.1423-0410.1994.tb00315.x
  2. Donskov SI, Morokov VR. Gruppy krovi cheloveka: ruk po immunoserologii. Moscow, RF: Binom; 2014. 1016 p. http://www.booksmed.com/allergologiya-immunologiya/3145-gruppy-krovi-cheloveka-donskov-si.html (In Russ.)
  3. Subramaniyan R. Diminished expression of B antigen mimicking B3 phenotype in a patient with AML-M3: a rare case report. Rev Bras Hematol Hemoter. 2016 Jul-Sep;38(3):264-66. doi: 10.1016/j.bjhh.2016.05.008
  4. Brecher ME, ed. Technical Manual. 14th ed. Bechesda, MD: American Association of Blood Banks; 2002. p. 667.
  5. Bryant N. An Introduction to immunohematology. 3rd ed. Philadelphia: W.B. Saunders Co; 1994. p. 115-16, 136. doi: 10.1111/j.1537-2995.1994.tb06021.x
  6. Motghare P, Kale L, Bedia AS, Charde S. Efficacy and accuracy of ABO blood group determination from saliva. JIAOMR. 2011;23(3):163-67. doi: 10.5005/jp-journals-10011-1120
  7. Noda H, Yokota M, Tatsumi S, Sugiyama S. Determination of ABO blood grouping from human oral squamous epithelium by the highly sensitive immunohistochemical staining method EnVision+. J Forensic Sci. 2002 Mar;47(2):341-44. doi: 10.1520/JFS15254J
  8. Denomme GA, Rios M, Rcid ME. Molecular Protocols in Transfusion Medicine. London, United Kingdom: Academic Press Publications; 2000. 186 p. https://www.elsevier.com/books/molecular-protocols-in-transfusion-medicine/denomme/978-0-12-209370-8
  9. Zhu Z, Ye L, Li Q, Gao H, Tan Y, Cai W. Red Cell Immunohematology research conducted in China. Transfus Med Rev. 2017 Apr;31(2):102-106. doi: 10.1016/j.tmrv.2016.11.004
  10. Chun S, Choi S, Yu H, Cho D. Cis-AB, the Blood Group of Many Faces, Is a Conundrum to the Novice Eye. Ann Lab Med. 2019 Mar;39(2):115-20. doi: 10.3343/alm.2019.39.2.115
  11. Mohammadi S, Moghaddam M, Babahajian S, Karimian MS, Ferdowsi S. Discrepancy in ABO Blood Grouping in a Blood Donor: a case report. IJBC. 2018; 10(2):61-63. http://ijbc.ir/article-1-795-en.pdf
Address for correspondence:
61022, Ukraine,
Kharkiv, Nauky Ave.,4,
Kharkiv National Medical University,
Department of Internal Medicine 2,
of Clinical Immunology and Allergology
named after Academician L.T.Malaya.
Tel. +380688888201,
e-mail: valentinka_1987@ukr.net,
Dielievska Valentyna Yuriivna
Information about the authors:
Kravchun Pavlo G., MD, Professor, Head of the Department of Internal Medicine 2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
https://orcid.org/0000-0002-8285-6763
Kozhyn Mykhailo I., PhD, Associate Professor of the Department of Internal Medicine 2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
https://orcid.org/0000-0002-8359-8890
Leontyeva Frida S., PhD, Head of the Department of Laboratory Diagnostics and Immunology, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0001-9801-7908
Vorontsov Petr M., PhD, Head of the Department of Experimental Modeling and Transplantology, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-5758-7223
Ashukina Nataliya A., PhD, Head of the Morphology of Connective Tissue, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-0478-7440
Marushchak Oleksii P., Orthopedist and Traumatologist, Institute of Spine and Joint Pathology named after M. I. Sytenko, Kharkiv, Ukraine.
https://orcid.org/0000-0002-5606-8763
Dielievska Valentyna Y., PhD, Assistant of the Department of Internal Medicine 2, of Clinical Immunology and Allergology Named After Academician L.T.Malaya, Kharkiv National Medical University, Kharkiv, Ukraine.
Contacts | ©Vitebsk State Medical University, 2007